Detailed information of TA system TA047352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 704470..705113
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: GS11_RS03180
• Protein ID: WP_003403187.1
• Coordinates: 704712..705113 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: GS11_RS03175
• Protein ID: WP_003403184.1
• Coordinates: 704470..704715 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS03140	699750..700280	-	531	WP_003917320.1	two component system sensor kinase HK2	-
GS11_RS03145	700264..700959	-	696	WP_197044064.1	response regulator transcription factor	-
GS11_RS03150	701082..701393	+	312	WP_003403164.1	hypothetical protein	-
GS11_RS03155	701465..702415	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
GS11_RS03160	702656..703240	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
GS11_RS03165	703242..703955	+	714	Protein_626	IS607 family element transposase accessory protein TnpB	-
GS11_RS03170	703955..704425	+	471	WP_003898523.1	hypothetical protein	-
GS11_RS03175	704470..704715	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GS11_RS03180	704712..705113	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
GS11_RS21915	705501..705668	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
GS11_RS21920	705701..705898	-	198	WP_003403191.1	hypothetical protein	-
GS11_RS03195	705978..707135	-	1158	WP_003403193.1	hypothetical protein	-
GS11_RS03200	707187..707408	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
GS11_RS03205	707550..708155	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T47352 WP_003403187.1 NZ_CP008744:704712-705113 [Mycobacterium tuberculosis variant bovis BCG]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T47352 NZ_CP008744:704712-705113 [Mycobacterium tuberculosis variant bovis BCG]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT47352 WP_003403184.1 NZ_CP008744:704470-704715 [Mycobacterium tuberculosis variant bovis BCG]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT47352 NZ_CP008744:704470-704715 [Mycobacterium tuberculosis variant bovis BCG]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91