Detailed information of TA system TA047351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 698380..699026
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: GS11_RS03125
• Protein ID: WP_003403137.1
• Coordinates: 698380..698793 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: GS11_RS03130
• Protein ID: WP_003403139.1
• Coordinates: 698790..699026 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS03105	694463..696013	+	1551	WP_003403119.1	MCE family protein	-
GS11_RS03110	696065..696457	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
GS11_RS03115	696454..696711	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
GS11_RS03120	696894..698129	-	1236	WP_003403128.1	ATP-binding protein	-
GS11_RS03125	698380..698793	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
GS11_RS03130	698790..699026	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GS11_RS03135	699130..699636	-	507	WP_003900973.1	two component system sensor kinase HK1	-
GS11_RS03140	699750..700280	-	531	WP_003917320.1	two component system sensor kinase HK2	-
GS11_RS03145	700264..700959	-	696	WP_197044064.1	response regulator transcription factor	-
GS11_RS03150	701082..701393	+	312	WP_003403164.1	hypothetical protein	-
GS11_RS03155	701465..702415	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
GS11_RS03160	702656..703240	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
GS11_RS03165	703242..703955	+	714	Protein_626	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T47351 WP_003403137.1 NZ_CP008744:c698793-698380 [Mycobacterium tuberculosis variant bovis BCG]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T47351 NZ_CP008744:c698793-698380 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT47351 WP_003403139.1 NZ_CP008744:c699026-698790 [Mycobacterium tuberculosis variant bovis BCG]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT47351 NZ_CP008744:c699026-698790 [Mycobacterium tuberculosis variant bovis BCG]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89