Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 696065..696711 Replicon chromosome
Accession NZ_CP008744
Organism Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag GS11_RS03110 Protein ID WP_003403122.1
Coordinates 696065..696457 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag GS11_RS03115 Protein ID WP_003403125.1
Coordinates 696454..696711 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS11_RS03095 691725..693253 + 1529 Protein_612 virulence factor Mce family protein -
GS11_RS03100 693316..694458 + 1143 WP_003911253.1 virulence factor Mce family protein -
GS11_RS03105 694463..696013 + 1551 WP_003403119.1 MCE family protein -
GS11_RS03110 696065..696457 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
GS11_RS03115 696454..696711 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
GS11_RS03120 696894..698129 - 1236 WP_003403128.1 ATP-binding protein -
GS11_RS03125 698380..698793 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
GS11_RS03130 698790..699026 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
GS11_RS03135 699130..699636 - 507 WP_003900973.1 two component system sensor kinase HK1 -
GS11_RS03140 699750..700280 - 531 WP_003917320.1 two component system sensor kinase HK2 -
GS11_RS03145 700264..700959 - 696 WP_197044064.1 response regulator transcription factor -
GS11_RS03150 701082..701393 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T47350 WP_003403122.1 NZ_CP008744:c696457-696065 [Mycobacterium tuberculosis variant bovis BCG]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T47350 NZ_CP008744:c696457-696065 [Mycobacterium tuberculosis variant bovis BCG]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT47350 WP_003403125.1 NZ_CP008744:c696711-696454 [Mycobacterium tuberculosis variant bovis BCG]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT47350 NZ_CP008744:c696711-696454 [Mycobacterium tuberculosis variant bovis BCG]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References