Detailed information of TA system TA047346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 364780..365351
• Replicon: chromosome
• Accession: NZ_CP008744
• Organism: Mycobacterium tuberculosis variant bovis BCG strain 3281

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: GS11_RS01580
• Protein ID: WP_003401560.1
• Coordinates: 364780..365082 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: GS11_RS01585
• Protein ID: WP_003401563.1
• Coordinates: 365130..365351 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS11_RS01560	360204..361007	-	804	WP_003401540.1	hypothetical protein	-
GS11_RS01565	361017..362414	-	1398	WP_003401544.1	sulfatase	-
GS11_RS01570	362593..364413	+	1821	WP_003401546.1	PE family protein	-
GS11_RS01575	364556..364783	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
GS11_RS01580	364780..365082	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
GS11_RS01585	365130..365351	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
GS11_RS01590	365348..365773	+	426	WP_003401566.1	PIN domain nuclease	-
GS11_RS01595	365910..366542	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
GS11_RS01600	366539..367447	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T47346 WP_003401560.1 NZ_CP008744:364780-365082 [Mycobacterium tuberculosis variant bovis BCG]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T47346 NZ_CP008744:364780-365082 [Mycobacterium tuberculosis variant bovis BCG]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT47346 WP_003401563.1 NZ_CP008744:365130-365351 [Mycobacterium tuberculosis variant bovis BCG]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT47346 NZ_CP008744:365130-365351 [Mycobacterium tuberculosis variant bovis BCG]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48