Detailed information of TA system TA047338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/GNAT-DUF1778
• Location: 59013..59767
• Replicon: chromosome
• Accession: NZ_CP008743
• Organism: Candidatus Nucleicultrix amoebiphila FS5

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: GQ61_RS00305
• Protein ID: WP_085783396.1
• Coordinates: 59258..59767 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: GQ61_RS00300
• Protein ID: WP_085783395.1
• Coordinates: 59013..59270 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ61_RS00280	54785..55432	-	648	WP_085783391.1	glutathione S-transferase N-terminal domain-containing protein	-
GQ61_RS00285	55438..55605	-	168	WP_085783392.1	zinc-finger domain-containing protein	-
GQ61_RS00290	55631..56995	-	1365	WP_085783393.1	MFS transporter	-
GQ61_RS00295	57119..58612	+	1494	WP_085783394.1	aldehyde dehydrogenase family protein	-
GQ61_RS00300	59013..59270	+	258	WP_085783395.1	DUF1778 domain-containing protein	Antitoxin
GQ61_RS00305	59258..59767	+	510	WP_085783396.1	GNAT family N-acetyltransferase	Toxin
GQ61_RS00310	59935..60465	+	531	WP_157111090.1	hypothetical protein	-
GQ61_RS00315	60482..62434	-	1953	WP_198157344.1	acetoacetate--CoA ligase	-
GQ61_RS00320	62570..63133	+	564	WP_085783399.1	hypothetical protein	-
GQ61_RS00325	63158..64351	+	1194	WP_085783400.1	CCA tRNA nucleotidyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-78)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18630.95 Da
• Isoelectric Point: 9.6028

>T47338 WP_085783396.1 NZ_CP008743:59258-59767 [Candidatus Nucleicultrix amoebiphila FS5]
MGKIEAPTPIHEKMILKDFSCGKVQLDIWLQKRALANERSGASRTYVLCKGGKAIAYYALCNGAIVHELASGKIKRNMPD
PIPAMIIARLAVDQNYHKQGIGKALLKDGILRILKASKIAGIRVILVHALDLDAQKFYKSVGFEESPLEELTLMITIHEA
LSSFKTSDD

Nucleotide

• Length: 510 bp

>T47338 NZ_CP008743:59258-59767 [Candidatus Nucleicultrix amoebiphila FS5]
ATGGGAAAAATAGAAGCACCTACGCCTATCCATGAAAAAATGATCCTTAAGGATTTTTCTTGTGGCAAAGTGCAGTTAGA
CATTTGGCTTCAAAAAAGGGCTCTGGCTAATGAACGTAGTGGTGCTTCAAGAACTTATGTTTTATGTAAAGGCGGTAAGG
CTATAGCTTATTATGCACTTTGTAATGGGGCTATTGTGCATGAACTTGCCTCAGGAAAAATTAAAAGAAACATGCCTGAT
CCAATTCCTGCAATGATTATTGCACGTTTGGCAGTTGATCAAAATTATCATAAGCAAGGGATTGGAAAAGCTCTTTTAAA
AGATGGAATCTTGCGTATTCTAAAAGCATCAAAAATTGCCGGTATTAGAGTGATTCTGGTGCATGCTTTAGATCTCGATG
CTCAAAAATTTTACAAATCTGTCGGATTTGAAGAATCCCCTTTAGAGGAGTTAACTCTCATGATAACTATTCATGAAGCT
TTAAGTTCATTTAAAACATCAGATGATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9819.21 Da
• Isoelectric Point: 8.6009

>AT47338 WP_085783395.1 NZ_CP008743:59013-59270 [Candidatus Nucleicultrix amoebiphila FS5]
MSQMNLRIDTARKNLIDHAAALQGKTRTDFMIEASYQAAEEVILDQRLFVLNDEQWTKFNQALDKAPSDNKKLRKLLNTA
APWEK

Nucleotide

• Length: 258 bp

>AT47338 NZ_CP008743:59013-59270 [Candidatus Nucleicultrix amoebiphila FS5]
ATGTCTCAGATGAATCTTAGAATCGACACAGCGAGAAAAAATCTTATAGATCATGCAGCTGCTTTACAAGGCAAGACACG
AACAGATTTTATGATTGAAGCTTCTTATCAAGCAGCAGAAGAAGTAATTTTAGATCAAAGATTATTTGTTCTGAATGATG
AGCAGTGGACTAAGTTCAATCAAGCCCTGGACAAGGCACCTTCTGATAACAAAAAACTGAGAAAACTCCTTAATACAGCT
GCTCCATGGGAAAAATAG