Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 1076072..1076739 Replicon chromosome
Accession NZ_CP008742
Organism Pseudomonas savastanoi pv. savastanoi NCPPB 3335

Toxin (Protein)


Gene name vapC Uniprot ID A0A0Q0GBP3
Locus tag PSA3335_RS05315 Protein ID WP_002556038.1
Coordinates 1076072..1076488 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID E7PUB4
Locus tag PSA3335_RS05320 Protein ID WP_002556039.1
Coordinates 1076485..1076739 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSA3335_RS05280 1071185..1072008 + 824 Protein_950 DUF945 domain-containing protein -
PSA3335_RS05285 1072068..1072268 + 201 WP_082301235.1 hypothetical protein -
PSA3335_RS05290 1072335..1072613 - 279 WP_004661031.1 mobilization protein -
PSA3335_RS05295 1072657..1072938 - 282 WP_011106972.1 hypothetical protein -
PSA3335_RS05300 1072911..1073255 - 345 WP_002556035.1 hypothetical protein -
PSA3335_RS05305 1073515..1073844 + 330 WP_002556036.1 ribbon-helix-helix protein, CopG family -
PSA3335_RS05310 1073855..1075810 + 1956 WP_002556037.1 relaxase/mobilization nuclease domain-containing protein -
PSA3335_RS05315 1076072..1076488 - 417 WP_002556038.1 type II toxin-antitoxin system VapC family toxin Toxin
PSA3335_RS05320 1076485..1076739 - 255 WP_002556039.1 plasmid stabilization protein Antitoxin
PSA3335_RS05325 1077086..1077946 + 861 WP_002556040.1 MurR/RpiR family transcriptional regulator -
PSA3335_RS05330 1077984..1078523 + 540 WP_004642348.1 D-alanyl-D-alanine dipeptidase -
PSA3335_RS05335 1078640..1078924 + 285 Protein_961 ABC transporter substrate-binding protein -
PSA3335_RS05340 1078976..1079808 + 833 Protein_962 IS3-like element IS51 family transposase -
PSA3335_RS05345 1079916..1080950 + 1035 WP_015060660.1 IS110-like element ISPsy16 family transposase -
PSA3335_RS05350 1081103..1081402 - 300 WP_082301236.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14865.14 Da        Isoelectric Point: 4.6579

>T47335 WP_002556038.1 NZ_CP008742:c1076488-1076072 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MIVLDTNVVSEAMKPESHLAVRAWLNDQAAETLYLSSVTLAELLFGIGALPAGKRKDMLAQALDGLMGLFRDRVLPFDID
AARRYADLAVTAKTCGRGFPTPDGYIAAIAASRDFIVASRDTAPYEAVGVPVINPWEV

Download         Length: 417 bp

>T47335 NZ_CP008742:c1076488-1076072 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGATCGTCCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGTCCCACTTGGCCGTGCGGGCCTGGCTGAACGA
TCAAGCCGCCGAAACGCTATACCTGTCCAGCGTGACGCTGGCCGAGCTGCTGTTTGGCATCGGGGCGCTCCCGGCCGGCA
AGCGCAAGGATATGCTGGCGCAGGCCCTTGACGGTCTAATGGGGCTGTTCAGGGATCGGGTGCTGCCATTCGATATCGAT
GCAGCACGGCGCTATGCCGATCTGGCCGTGACGGCAAAAACCTGCGGGCGAGGATTCCCGACACCTGACGGCTACATCGC
AGCGATTGCGGCATCGCGAGATTTCATCGTGGCGTCACGCGACACTGCACCCTATGAGGCGGTCGGTGTACCCGTCATCA
ATCCGTGGGAAGTGTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9442.84 Da        Isoelectric Point: 7.3219

>AT47335 WP_002556039.1 NZ_CP008742:c1076739-1076485 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MAMLTVRNLPDDVHRALRVRAAQHGHSTEAEVREILAIAVKPETRVRLGEALAALGRKIGLTNEDFEVFNQVRDKTPAEP
LRFE

Download         Length: 255 bp

>AT47335 NZ_CP008742:c1076739-1076485 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGGCAATGCTGACAGTACGGAACTTGCCCGACGATGTACACCGCGCGCTACGAGTGCGGGCCGCCCAACACGGGCACAG
CACCGAGGCCGAAGTCCGCGAGATTCTGGCGATCGCAGTCAAGCCGGAGACGCGCGTTCGCCTAGGTGAAGCCTTGGCGG
CATTGGGTCGCAAGATCGGCCTGACGAACGAAGATTTCGAGGTCTTCAACCAAGTGCGAGACAAGACGCCGGCCGAGCCG
CTGAGGTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0GBP3

Antitoxin

Source ID Structure
AlphaFold DB A0A2G9L5L9

References