Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 593426..594037 Replicon chromosome
Accession NZ_CP008742
Organism Pseudomonas savastanoi pv. savastanoi NCPPB 3335

Toxin (Protein)


Gene name PfiT Uniprot ID A0A554GDS8
Locus tag PSA3335_RS02920 Protein ID WP_002556077.1
Coordinates 593687..594037 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q4ZME7
Locus tag PSA3335_RS02915 Protein ID WP_002556076.1
Coordinates 593426..593674 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSA3335_RS02890 589247..589542 - 296 Protein_515 DDE-type integrase/transposase/recombinase -
PSA3335_RS02895 589802..590242 + 441 WP_044319945.1 hypothetical protein -
PSA3335_RS02900 590641..591157 + 517 Protein_517 GAF domain-containing protein -
PSA3335_RS02905 591497..592027 + 531 WP_031598249.1 hypothetical protein -
PSA3335_RS02910 592129..593088 - 960 WP_002556075.1 tyrosine-type recombinase/integrase -
PSA3335_RS02915 593426..593674 + 249 WP_002556076.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
PSA3335_RS02920 593687..594037 + 351 WP_002556077.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PSA3335_RS02925 594131..594535 - 405 WP_031598244.1 hypothetical protein -
PSA3335_RS02930 594525..597347 - 2823 WP_031598243.1 RHS repeat-associated core domain-containing protein -
PSA3335_RS02935 597344..598465 - 1122 WP_082301218.1 RHS repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - algB 358770..598620 239850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13011.90 Da        Isoelectric Point: 4.4055

>T47334 WP_002556077.1 NZ_CP008742:593687-594037 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MNTFALRFTDVAQQSLEDQVEHLAVHQGFSSAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITFIVILLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T47334 NZ_CP008742:593687-594037 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGCT
ACCCGGTCAGCCCTCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGATGGATTCAGATGGTATTACCTTCATCGTCATATTGCTCGTATTAGGAGGTAAACAAAGTGTTGAACAAGCCCT
CATCCGCTATTGCCTTCTTCAGCCTATATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9366.73 Da        Isoelectric Point: 9.9579

>AT47334 WP_002556076.1 NZ_CP008742:593426-593674 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MRVETISFLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFT
QK

Download         Length: 249 bp

>AT47334 NZ_CP008742:593426-593674 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCATTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A554GDS8

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9GM89

References