Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 529386..530059 Replicon chromosome
Accession NZ_CP008742
Organism Pseudomonas savastanoi pv. savastanoi NCPPB 3335

Toxin (Protein)


Gene name vapC Uniprot ID H1ZWW2
Locus tag PSA3335_RS02545 Protein ID WP_002556053.1
Coordinates 529640..530059 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48B52
Locus tag PSA3335_RS02540 Protein ID WP_003381346.1
Coordinates 529386..529643 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSA3335_RS02505 524521..524742 + 222 Protein_453 helix-turn-helix transcriptional regulator -
PSA3335_RS28935 524743..524820 + 78 Protein_454 DUF159 family protein -
PSA3335_RS02510 524923..526152 - 1230 WP_082301212.1 IS91 family transposase -
PSA3335_RS02520 526545..527288 + 744 WP_002556041.1 hypothetical protein -
PSA3335_RS02525 527285..528097 + 813 WP_002556042.1 DUF3037 domain-containing protein -
PSA3335_RS02535 528339..529112 - 774 WP_002556043.1 hypothetical protein -
PSA3335_RS02540 529386..529643 + 258 WP_003381346.1 Arc family DNA-binding protein Antitoxin
PSA3335_RS02545 529640..530059 + 420 WP_002556053.1 type II toxin-antitoxin system VapC family toxin Toxin
PSA3335_RS02550 530100..530732 + 633 WP_015060648.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - algB 358770..598620 239850
- flank IS/Tn - - 524923..526131 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Arc-like DNA binding domain

(5-46)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15168.46 Da        Isoelectric Point: 4.7010

>T47333 WP_002556053.1 NZ_CP008742:529640-530059 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWET

Download         Length: 420 bp

>T47333 NZ_CP008742:529640-530059 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTAGAAACGCTTTATCTGTCCACCATCACCGTAGCCGAGTTGCGTGCAGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAACGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9401.62 Da        Isoelectric Point: 9.0330

>AT47333 WP_003381346.1 NZ_CP008742:529386-529643 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Download         Length: 258 bp

>AT47333 NZ_CP008742:529386-529643 [Pseudomonas savastanoi pv. savastanoi NCPPB 3335]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCCATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACATACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M2ZA27

Antitoxin

Source ID Structure
AlphaFold DB Q48B52

References