Detailed information of TA system TA047319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147638..148143
• Replicon: chromosome
• Accession: NZ_CP008739
• Organism: Pseudomonas aeruginosa VRFPA04

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: P797_RS00655
• Protein ID: WP_003083773.1
• Coordinates: 147638..147919 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: P797_RS00660
• Protein ID: WP_003083775.1
• Coordinates: 147916..148143 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P797_RS00630	142888..144237	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
P797_RS00635	144286..144972	+	687	WP_003083762.1	FadR family transcriptional regulator	-
P797_RS00640	145073..145792	+	720	Protein_130	GntR family transcriptional regulator	-
P797_RS00645	146012..146398	+	387	WP_003083767.1	aegerolysin family protein	-
P797_RS00650	146430..147338	-	909	WP_003083769.1	LysR family transcriptional regulator	-
P797_RS00655	147638..147919	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
P797_RS00660	147916..148143	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
P797_RS00665	148320..148943	-	624	WP_019371309.1	hypothetical protein	-
P797_RS00670	149044..149544	+	501	WP_003101228.1	LEA type 2 family protein	-
P797_RS00675	149617..149958	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
P797_RS00680	150040..151296	-	1257	WP_072017998.1	GABA permease	-
P797_RS31780	151283..151453	+	171	Protein_139	cytochrome c oxidase copper chaperone SenC	-
P797_RS00690	151624..152097	+	474	WP_003083753.1	helix-turn-helix transcriptional regulator	-
P797_RS00695	152352..152810	+	459	Protein_141	amino acid permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T47319 WP_003083773.1 NZ_CP008739:c147919-147638 [Pseudomonas aeruginosa VRFPA04]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T47319 NZ_CP008739:c147919-147638 [Pseudomonas aeruginosa VRFPA04]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT47319 WP_003083775.1 NZ_CP008739:c148143-147916 [Pseudomonas aeruginosa VRFPA04]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT47319 NZ_CP008739:c148143-147916 [Pseudomonas aeruginosa VRFPA04]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9