Detailed information of TA system TA047313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1595729..1596285
• Replicon: chromosome
• Accession: NZ_CP008730
• Organism: Burkholderia multivorans strain DDS 15A-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8A8D2I2
• Locus tag: DM80_RS23210
• Protein ID: WP_034188048.1
• Coordinates: 1595995..1596285 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8A8D2B6
• Locus tag: DM80_RS23205
• Protein ID: WP_025369732.1
• Coordinates: 1595729..1596007 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM80_RS23185	1591312..1592463	-	1152	WP_048805412.1	efflux RND transporter periplasmic adaptor subunit	-
DM80_RS23190	1592460..1593068	-	609	WP_004393990.1	TetR/AcrR family transcriptional regulator	-
DM80_RS23195	1593210..1593470	-	261	WP_025369735.1	hypothetical protein	-
DM80_RS34600	1594034..1594303	+	270	Protein_1443	mobilization protein	-
DM80_RS23200	1594464..1595666	+	1203	WP_048805413.1	hypothetical protein	-
DM80_RS23205	1595729..1596007	+	279	WP_025369732.1	CopG family ribbon-helix-helix protein	Antitoxin
DM80_RS23210	1595995..1596285	+	291	WP_034188048.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DM80_RS34605	1596282..1596521	+	240	WP_144245534.1	hypothetical protein	-
DM80_RS23215	1596559..1597551	-	993	WP_006412423.1	IS5-like element ISBmu2 family transposase	-
DM80_RS36375	1597655..1597804	+	150	WP_158332655.1	hypothetical protein	-
DM80_RS23220	1597884..1598159	+	276	WP_200885124.1	hypothetical protein	-
DM80_RS23225	1598208..1598468	-	261	Protein_1451	hypothetical protein	-
DM80_RS23230	1598688..1600199	+	1512	WP_027809980.1	IS21 family transposase	-
DM80_RS23235	1600204..1600992	+	789	WP_012213993.1	IS21-like element ISBmu1 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	1505074..1610035	104961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10968.49 Da
• Isoelectric Point: 9.5558

>T47313 WP_034188048.1 NZ_CP008730:1595995-1596285 [Burkholderia multivorans]
MPQVIFAPAAIRDLQRLRDFLRPKNPDAARRAGEAIRQGVQALGTHPRMGRLVEDLPEHYREWPIDFGDSGYVARYRLDG
ETVTILAVRHQKEAGY

Nucleotide

• Length: 291 bp

>T47313 NZ_CP008730:1595995-1596285 [Burkholderia multivorans]
ATGCCGCAAGTGATCTTTGCGCCGGCGGCAATCCGAGACTTGCAACGGTTGCGCGACTTCCTGCGCCCGAAGAACCCTGA
CGCCGCGAGGCGGGCCGGCGAAGCAATTAGACAAGGCGTACAGGCTCTCGGCACCCACCCTCGAATGGGGCGTCTTGTAG
AAGACCTACCCGAGCATTATCGGGAGTGGCCCATCGATTTCGGGGATAGTGGTTACGTGGCCCGGTACCGCCTCGATGGC
GAAACCGTTACGATCTTGGCCGTCCGACATCAGAAGGAAGCGGGGTATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10820.15 Da
• Isoelectric Point: 5.2484

>AT47313 WP_025369732.1 NZ_CP008730:1595729-1596007 [Burkholderia multivorans]
MVTATSIKLDDELKGRVQHLAEARRRTPHWIMREAIEQYVEREEKRETLNKDTLKAWDEFQATGLHATAEEVDKWLASWG
TENELPTPECRK

Nucleotide

• Length: 279 bp

>AT47313 NZ_CP008730:1595729-1596007 [Burkholderia multivorans]
ATGGTAACAGCGACATCTATCAAGCTCGATGACGAACTGAAAGGGCGAGTACAGCACTTGGCTGAAGCGCGTCGGCGAAC
CCCGCACTGGATCATGCGCGAAGCCATCGAGCAGTACGTCGAGCGCGAGGAAAAGCGGGAGACGTTGAACAAGGACACGC
TCAAGGCGTGGGATGAGTTTCAGGCGACTGGTCTACACGCGACCGCTGAAGAAGTGGATAAGTGGCTCGCGAGCTGGGGA
ACTGAAAACGAGCTGCCCACGCCCGAATGCCGCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A8A8D2I2

Antitoxin

• AlphaFold DB: A0A8A8D2B6