Detailed information of TA system TA047309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2118002..2118693
• Replicon: chromosome
• Accession: NZ_CP008729
• Organism: Burkholderia multivorans strain DDS 15A-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DM80_RS14165
• Protein ID: WP_048804531.1
• Coordinates: 2118238..2118693 (+)
• Length: 152 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A132F1C7
• Locus tag: DM80_RS14160
• Protein ID: WP_012216644.1
• Coordinates: 2118002..2118241 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM80_RS14135	2113368..2113607	-	240	WP_006416205.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DM80_RS14140	2114013..2114696	-	684	WP_035955277.1	Fe2+-dependent dioxygenase	-
DM80_RS14145	2114693..2115448	-	756	WP_048804528.1	sel1 repeat family protein	-
DM80_RS14150	2115452..2117698	-	2247	WP_048804529.1	TonB-dependent siderophore receptor	-
DM80_RS14160	2118002..2118241	+	240	WP_012216644.1	Arc family DNA-binding protein	Antitoxin
DM80_RS14165	2118238..2118693	+	456	WP_048804531.1	type II toxin-antitoxin system VapC family toxin	Toxin
DM80_RS14170	2118791..2120086	-	1296	WP_012216646.1	aspartate carbamoyltransferase	-
DM80_RS14175	2120319..2120453	+	135	WP_006396654.1	entericidin A/B family lipoprotein	-
DM80_RS14180	2120549..2121124	-	576	WP_048804532.1	hypothetical protein	-
DM80_RS14185	2121335..2122285	-	951	WP_080746542.1	DMT family transporter	-
DM80_RS14190	2122781..2123575	-	795	WP_048804534.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 152 a.a.
• Molecular weight: 16064.40 Da
• Isoelectric Point: 7.2060

>T47309 WP_048804531.1 NZ_CP008729:2118238-2118693 [Burkholderia multivorans]
MILVDTNVIAEPLRREPSAAVIEWLDAQNVETLFLAAISLAEMRFGVAALPEGRRRDWLAQSIEHRVVPVFRGRILPFDD
AASKAYARIRAHARAGGNAIAPADGYIAATAEANGLIVATRDVAPFEAAGLRVIDPGASQGAPVRQGIASK

Nucleotide

• Length: 456 bp

>T47309 NZ_CP008729:2118238-2118693 [Burkholderia multivorans]
ATGATCCTTGTTGACACGAACGTCATTGCCGAACCGCTGCGGCGCGAGCCGAGCGCGGCCGTGATCGAATGGCTCGACGC
ACAGAACGTCGAGACGCTGTTCCTGGCCGCGATCAGCCTCGCCGAAATGCGCTTCGGTGTCGCGGCGCTGCCGGAAGGGC
GCCGGCGGGACTGGCTGGCGCAAAGCATCGAGCATCGTGTCGTGCCGGTGTTTCGCGGCCGGATCCTGCCGTTCGACGAT
GCCGCGAGCAAGGCCTATGCGCGCATTCGCGCGCACGCCCGTGCCGGCGGCAACGCGATCGCGCCCGCCGACGGCTACAT
TGCCGCGACGGCCGAGGCGAACGGCCTGATCGTCGCGACGCGCGACGTCGCGCCGTTCGAGGCCGCGGGCCTGCGCGTGA
TCGATCCGGGGGCGTCGCAGGGCGCGCCGGTGCGCCAAGGTATCGCGTCGAAATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8716.94 Da
• Isoelectric Point: 5.1133

>AT47309 WP_012216644.1 NZ_CP008729:2118002-2118241 [Burkholderia multivorans]
MPVITVRNLPDEVHRALRVRAALHGRSTEAEVRDILEQAVLSEGRVKLGTLLAEIGREAGGVDLDIQRDKTPTDPMSFE

Nucleotide

• Length: 240 bp

>AT47309 NZ_CP008729:2118002-2118241 [Burkholderia multivorans]
ATGCCGGTGATTACCGTTCGAAATTTGCCGGATGAAGTGCATCGCGCCCTTCGGGTCCGCGCGGCGCTGCACGGGCGCAG
TACGGAGGCAGAGGTGCGCGACATCCTCGAGCAGGCCGTGTTGTCGGAAGGGCGCGTGAAGCTCGGAACCTTGCTGGCGG
AAATCGGGCGGGAGGCAGGCGGTGTCGATCTCGACATTCAGCGTGACAAGACGCCAACCGATCCGATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A132F1C7