Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 368357..368934 Replicon chromosome
Accession NZ_CP008729
Organism Burkholderia multivorans strain DDS 15A-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A2S9ML41
Locus tag DM80_RS06560 Protein ID WP_035954580.1
Coordinates 368569..368934 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2S9ML45
Locus tag DM80_RS06555 Protein ID WP_035956044.1
Coordinates 368357..368575 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DM80_RS06535 363999..365270 - 1272 WP_048803763.1 Zn-dependent hydrolase -
DM80_RS06540 365263..365799 - 537 WP_048803764.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
DM80_RS06545 366017..367183 - 1167 WP_048803765.1 branched-chain amino acid ABC transporter substrate-binding protein -
DM80_RS06550 367359..368255 + 897 WP_048803766.1 LysR family transcriptional regulator -
DM80_RS06555 368357..368575 + 219 WP_035956044.1 hypothetical protein Antitoxin
DM80_RS06560 368569..368934 + 366 WP_035954580.1 type II toxin-antitoxin system VapC family toxin Toxin
DM80_RS06565 368985..369419 - 435 WP_009688132.1 GNAT family N-acetyltransferase -
DM80_RS06570 369675..370238 + 564 WP_006399806.1 peroxiredoxin -
DM80_RS06575 370357..371955 + 1599 WP_048803767.1 alkyl hydroperoxide reductase subunit F -
DM80_RS06580 372016..372750 - 735 WP_181145705.1 hypothetical protein -
DM80_RS06585 372874..373773 - 900 WP_048803769.1 lipid A hydroxylase LpxO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13552.56 Da        Isoelectric Point: 4.8414

>T47306 WP_035954580.1 NZ_CP008729:368569-368934 [Burkholderia multivorans]
MVKALFDTNILIDYLSGVESARLELGRYDYRAISTITWMEVLVGTTADDEGPIRAWLSSFDVIPLDSTVADRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGIRVPYQV

Download         Length: 366 bp

>T47306 NZ_CP008729:368569-368934 [Burkholderia multivorans]
ATGGTAAAAGCTCTCTTCGACACCAACATCCTGATCGACTATCTGAGCGGCGTGGAGTCGGCCCGCCTGGAGCTCGGCCG
CTACGACTACCGTGCGATCAGCACCATTACGTGGATGGAAGTCCTGGTCGGTACGACTGCAGATGACGAAGGCCCGATTC
GCGCGTGGCTCTCGTCGTTCGACGTGATCCCGCTCGACAGTACGGTTGCCGACCGCGCCGTGACCATTCGCAAGGAACGG
CGCATTCGTCTGCCCGACGCGATCGTCTGGGCATCGGCCCAGGTAAACGGGCTGCTGCTCGTGTCGCGTAACACGAAGGA
CTTTCCGGCAACGGAACCCGGCATCCGGGTGCCTTATCAGGTCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8231.38 Da        Isoelectric Point: 4.7640

>AT47306 WP_035956044.1 NZ_CP008729:368357-368575 [Burkholderia multivorans]
MSRVLVDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRAVDGLAYQEKLRSEW

Download         Length: 219 bp

>AT47306 NZ_CP008729:368357-368575 [Burkholderia multivorans]
ATGAGCCGAGTCTTGGTGGATCTGTCGGACGGCCAGCTCGACGAGCTGGCCGTCATCGTCGAGACTGAGCATCGTCCTCG
CGCCGCGATCATTCGCGATGCGATTGACGCTTACATCGCCCTACACAAGCGCCCGCTCGCCGACGATGTCTTCGGTCTCT
GGAAAGACCGCGCCGTCGACGGGCTCGCTTATCAGGAAAAACTGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S9ML41

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9ML45

References