Detailed information of TA system TA047304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 622221..622816
• Replicon: chromosome
• Accession: NZ_CP008728
• Organism: Burkholderia multivorans strain DDS 15A-1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: A0A2A4C4A8
• Locus tag: DM80_RS02780
• Protein ID: WP_012218081.1
• Coordinates: 622221..622571 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A2A4C1X6
• Locus tag: DM80_RS02785
• Protein ID: WP_012218082.1
• Coordinates: 622565..622816 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM80_RS02760	617247..618095	-	849	WP_027813534.1	heteromeric transposase endonuclease subunit TnsA	-
DM80_RS35385	618361..618804	-	444	WP_143331194.1	hypothetical protein	-
DM80_RS02765	618814..619068	-	255	WP_038442290.1	hypothetical protein	-
DM80_RS32990	619124..620068	-	945	WP_012218077.1	hypothetical protein	-
DM80_RS35390	620282..620851	-	570	WP_012218078.1	hypothetical protein	-
DM80_RS35395	620875..621207	-	333	WP_096508413.1	hypothetical protein	-
DM80_RS36250	621292..621453	+	162	WP_155295622.1	hypothetical protein	-
DM80_RS02770	621450..621635	-	186	WP_038442441.1	hypothetical protein	-
DM80_RS02775	621698..622210	-	513	WP_012218080.1	hypothetical protein	-
DM80_RS02780	622221..622571	-	351	WP_012218081.1	type II toxin-antitoxin system ChpB family toxin	Toxin
DM80_RS02785	622565..622816	-	252	WP_012218082.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DM80_RS02790	623008..623688	-	681	WP_012218083.1	site-specific integrase	-
DM80_RS02795	623962..624954	-	993	WP_007182917.1	IS5-like element ISBmu23 family transposase	-
DM80_RS02800	625067..625393	+	327	WP_012464905.1	H-NS histone family protein	-
DM80_RS02805	625524..626315	+	792	WP_012218085.1	SOS response-associated peptidase family protein	-
DM80_RS35400	626488..626682	-	195	WP_012464904.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	272754..863135	590381
	-	flank	IS/Tn	-	-	623962..624954	992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-115)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12420.35 Da
• Isoelectric Point: 8.9716

>T47304 WP_012218081.1 NZ_CP008728:c622571-622221 [Burkholderia multivorans]
MVKRVKFDRGDIVRVSLNRTIGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEARGARTIERAPAEVVEDALARLQSIIE

Nucleotide

• Length: 351 bp

>T47304 NZ_CP008728:c622571-622221 [Burkholderia multivorans]
ATGGTAAAGCGGGTCAAGTTCGATCGGGGCGACATCGTGCGCGTTAGCCTGAACCGGACCATCGGGCGCGAGCAGCAGGG
CGATTTCCGTCCCGCGCTTGTGCTGTCTCCCGCCGCATTCAATGCCTTGGGGGTGGCGCTCGTCGCGCCGATCACGCAGG
GCGGGGAGTTTGCGCGCTTCGCAGGATTCGCCGTGCCGCTCTCTGGCTCGGGCACCGAGACGCAGGGCGTGGCCTTGGTG
AACATGGTCCGGATGCTCGATCTGGAGGCGCGTGGCGCACGGACGATCGAGCGGGCTCCGGCCGAGGTGGTCGAAGATGC
GCTGGCTCGGCTGCAATCGATAATCGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8997.39 Da
• Isoelectric Point: 6.9770

>AT47304 WP_012218082.1 NZ_CP008728:c622816-622565 [Burkholderia multivorans]
MELKIQKWGNSAAVRLPAVLLEQMHASVGGSLNADVRPDGVLLTPARRKYALDELVAQCDAKAPFPADMAAWGEVRPVGR
EGW

Nucleotide

• Length: 252 bp

>AT47304 NZ_CP008728:c622816-622565 [Burkholderia multivorans]
ATGGAACTAAAAATTCAGAAATGGGGCAACAGTGCGGCGGTGCGACTCCCCGCCGTGTTGCTCGAGCAGATGCACGCCTC
GGTCGGCGGCTCGCTGAACGCCGACGTGCGTCCGGACGGCGTATTGCTGACCCCCGCGCGCCGCAAGTATGCGCTGGACG
AACTGGTCGCTCAGTGTGATGCGAAAGCGCCTTTCCCGGCGGACATGGCTGCATGGGGTGAGGTCAGGCCGGTAGGGCGT
GAAGGATGGTAA

Structures

Toxin

• AlphaFold DB: A0A2A4C4A8

Antitoxin

• AlphaFold DB: A0A2A4C1X6