Detailed information of TA system TA047287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 50761..51320
• Replicon: plasmid pEC648_1
• Accession: NZ_CP008715
• Organism: Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: FH07_RS25245
• Protein ID: WP_000421272.1
• Coordinates: 50761..51036 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: FH07_RS25250
• Protein ID: WP_001387467.1
• Coordinates: 51036..51320 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH07_RS25215	46810..47550	-	741	WP_060684531.1	tyrosine-type recombinase/integrase	-
FH07_RS28675	47671..47853	-	183	WP_042344623.1	hypothetical protein	-
FH07_RS27315	48196..48503	-	308	Protein_51	type II toxin-antitoxin system ParD family antitoxin	-
FH07_RS25235	48996..50166	+	1171	Protein_52	hypothetical protein	-
FH07_RS28680	50199..50327	+	129	Protein_53	IS481 family transposase	-
FH07_RS25240	50362..50655	-	294	WP_001105060.1	hypothetical protein	-
FH07_RS25245	50761..51036	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FH07_RS25250	51036..51320	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
FH07_RS25255	51925..52677	+	753	WP_000562172.1	IclR family transcriptional regulator	-
FH07_RS25260	52723..53675	-	953	Protein_58	2-keto-3-deoxygluconate transporter	-
FH07_RS25265	53708..54088	-	381	WP_024946717.1	Rid family detoxifying hydrolase	-
FH07_RS25270	54113..55003	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
FH07_RS25275	55236..55430	-	195	WP_000796505.1	hypothetical protein	-
FH07_RS25280	55404..55844	+	441	Protein_62	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / tet(B)	iucA / iucB / iucC / iucD / iutA	1..120653	120653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T47287 WP_000421272.1 NZ_CP008715:c51036-50761 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T47287 NZ_CP008715:c51036-50761 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT47287 WP_001387467.1 NZ_CP008715:c51320-51036 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT47287 NZ_CP008715:c51320-51036 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880