Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4123023..4123855 Replicon chromosome
Accession NZ_CP008697
Organism Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)


Gene name yeeV Uniprot ID A0A6G4K9Y9
Locus tag FH07_RS20385 Protein ID WP_032333762.1
Coordinates 4123481..4123855 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NQ68
Locus tag FH07_RS20380 Protein ID WP_001540478.1
Coordinates 4123023..4123391 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH07_RS20340 4118856..4119536 + 681 WP_032333197.1 WYL domain-containing protein -
FH07_RS20345 4119684..4120361 + 678 WP_060684486.1 hypothetical protein -
FH07_RS20350 4120367..4120600 + 234 WP_001278283.1 DUF905 family protein -
FH07_RS20355 4120690..4121508 + 819 WP_001773857.1 DUF945 domain-containing protein -
FH07_RS20360 4121599..4122084 + 486 WP_000214398.1 antirestriction protein -
FH07_RS20365 4122100..4122576 + 477 WP_001186786.1 RadC family protein -
FH07_RS20370 4122639..4122860 + 222 WP_000692311.1 DUF987 domain-containing protein -
FH07_RS20380 4123023..4123391 + 369 WP_001540478.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
FH07_RS20385 4123481..4123855 + 375 WP_032333762.1 TA system toxin CbtA family protein Toxin
FH07_RS20390 4123852..4124340 + 489 WP_000777545.1 hypothetical protein -
FH07_RS20395 4124352..4124549 + 198 WP_000839281.1 DUF957 domain-containing protein -
FH07_RS20400 4124646..4125215 + 570 WP_001560692.1 DUF4942 domain-containing protein -
FH07_RS28880 4125573..4125728 + 156 WP_000729638.1 hypothetical protein -
FH07_RS20410 4126539..4127522 + 984 WP_024946586.1 KpsF/GutQ family sugar-phosphate isomerase -
FH07_RS20415 4127594..4128742 + 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papX 4051873..4125342 73469
- flank IS/Tn - - 4125573..4125728 155


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14017.06 Da        Isoelectric Point: 8.2905

>T47249 WP_032333762.1 NZ_CP008697:4123481-4123855 [Escherichia coli]
MKTLPDIHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T47249 NZ_CP008697:4123481-4123855 [Escherichia coli]
ATGAAAACATTACCCGATATACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13652.53 Da        Isoelectric Point: 7.8398

>AT47249 WP_001540478.1 NZ_CP008697:4123023-4123391 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT47249 NZ_CP008697:4123023-4123391 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G4K9Y9

Antitoxin

Source ID Structure
AlphaFold DB S1NQ68

References