Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2959952..2960783 Replicon chromosome
Accession NZ_CP008697
Organism Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag FH07_RS14760 Protein ID WP_000854814.1
Coordinates 2960409..2960783 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag FH07_RS14755 Protein ID WP_001285585.1
Coordinates 2959952..2960320 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH07_RS14725 2957056..2957511 + 456 WP_000581504.1 hypothetical protein -
FH07_RS14730 2957590..2957829 + 240 WP_060684439.1 DUF905 family protein -
FH07_RS14735 2957768..2958541 + 774 WP_060684440.1 DUF945 domain-containing protein -
FH07_RS14740 2958623..2959102 + 480 WP_000860087.1 antirestriction protein -
FH07_RS14745 2959118..2959594 + 477 WP_060684441.1 RadC family protein -
FH07_RS14750 2959657..2959878 + 222 WP_000692323.1 DUF987 domain-containing protein -
FH07_RS14755 2959952..2960320 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
FH07_RS14760 2960409..2960783 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
FH07_RS14765 2960780..2960974 + 195 WP_000988600.1 hypothetical protein -
FH07_RS28495 2960987..2961100 + 114 WP_001161660.1 DUF957 domain-containing protein -
FH07_RS26675 2961389..2961517 - 129 Protein_2813 transposase domain-containing protein -
FH07_RS14775 2961589..2961771 + 183 WP_001016348.1 hypothetical protein -
FH07_RS14780 2961872..2962201 - 330 WP_000450409.1 DUF496 family protein -
FH07_RS14785 2962373..2963431 - 1059 WP_001200891.1 FUSC family protein -
FH07_RS14790 2963629..2964102 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
FH07_RS14795 2964221..2965387 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T47245 WP_000854814.1 NZ_CP008697:2960409-2960783 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T47245 NZ_CP008697:2960409-2960783 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT47245 WP_001285585.1 NZ_CP008697:2959952-2960320 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT47245 NZ_CP008697:2959952-2960320 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References