Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1262230..1262848 Replicon chromosome
Accession NZ_CP008697
Organism Escherichia coli strain ST648 isolate Pleural Effusion of Patients with Empyema Thoracis

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FH07_RS06300 Protein ID WP_001291435.1
Coordinates 1262230..1262448 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FH07_RS06305 Protein ID WP_000344800.1
Coordinates 1262474..1262848 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH07_RS06265 1257519..1258091 + 573 WP_000779831.1 YbaY family lipoprotein -
FH07_RS06270 1258122..1258433 - 312 WP_000409911.1 MGMT family protein -
FH07_RS06280 1258812..1259165 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FH07_RS06285 1259207..1260757 - 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FH07_RS06290 1260921..1261391 - 471 WP_022645280.1 YlaC family protein -
FH07_RS06295 1261507..1262058 - 552 WP_000102574.1 maltose O-acetyltransferase -
FH07_RS06300 1262230..1262448 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FH07_RS06305 1262474..1262848 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FH07_RS06310 1263394..1266543 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FH07_RS06315 1266566..1267759 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T47233 WP_001291435.1 NZ_CP008697:c1262448-1262230 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T47233 NZ_CP008697:c1262448-1262230 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT47233 WP_000344800.1 NZ_CP008697:c1262848-1262474 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT47233 NZ_CP008697:c1262848-1262474 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References