Detailed information of TA system TA047223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 6077629..6078106
• Replicon: chromosome
• Accession: NZ_CP008696
• Organism: Pseudomonas chlororaphis strain PA23

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: EY04_RS27105
• Protein ID: WP_038635405.1
• Coordinates: 6077840..6078106 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: EY04_RS27100
• Protein ID: WP_038635403.1
• Coordinates: 6077629..6077856 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EY04_RS27075	6073331..6073921	+	591	WP_038635390.1	hypothetical protein	-
EY04_RS33515	6073918..6074685	+	768	WP_051673175.1	hypothetical protein	-
EY04_RS27085	6074678..6075643	+	966	WP_158484770.1	type III secretion system gatekeeper subunit SctW	-
EY04_RS27090	6075681..6076118	+	438	WP_051673177.1	type III secretion system chaperone	-
EY04_RS27095	6076259..6077311	+	1053	WP_038635400.1	hypothetical protein	-
EY04_RS27100	6077629..6077856	+	228	WP_038635403.1	CopG family transcriptional regulator	Antitoxin
EY04_RS27105	6077840..6078106	+	267	WP_038635405.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EY04_RS32765	6078393..6078536	-	144	Protein_5428	transposase domain-containing protein	-
EY04_RS32770	6078732..6079707	-	976	Protein_5429	IS3 family transposase	-
EY04_RS27120	6080689..6082545	+	1857	WP_144241864.1	hypothetical protein	-
EY04_RS27125	6082849..6083106	-	258	WP_038635415.1	EscE/YscE/SsaE family type III secretion system needle protein co-chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6063232..6084346	21114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10287.82 Da
• Isoelectric Point: 9.9949

>T47223 WP_038635405.1 NZ_CP008696:6077840-6078106 [Pseudomonas chlororaphis]
MAWTIDYTDTAKTQLRKLEKQSAKRILDFMDERVSSRDDPRTTGKALTGPLGGLWRYRVEDFRVICEIQDGALRILVVQL
GNRREVYR

Nucleotide

• Length: 267 bp

>T47223 NZ_CP008696:6077840-6078106 [Pseudomonas chlororaphis]
TTGGCCTGGACGATTGATTACACCGACACGGCCAAAACACAGTTGCGCAAGCTCGAAAAGCAGTCAGCGAAGCGCATTCT
TGATTTTATGGATGAGCGGGTGTCCAGCAGGGATGATCCGAGAACTACTGGCAAGGCGCTTACAGGTCCATTGGGTGGCT
TGTGGCGGTATCGCGTAGAAGACTTCCGAGTAATCTGCGAAATTCAGGACGGTGCCTTACGGATACTGGTAGTACAGCTT
GGCAACCGTCGAGAAGTTTACCGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8569.56 Da
• Isoelectric Point: 4.6263

>AT47223 WP_038635403.1 NZ_CP008696:6077629-6077856 [Pseudomonas chlororaphis]
MATSIRLAPETEQRLDFLATSTGRTKAYYLREIINHGLTDLEDYYLAAEVLERVRKNTEDVHSAAEVRKDLGLDD

Nucleotide

• Length: 228 bp

>AT47223 NZ_CP008696:6077629-6077856 [Pseudomonas chlororaphis]
ATGGCCACTTCCATTCGCTTGGCACCCGAAACCGAGCAGCGCTTAGATTTTTTGGCGACCAGCACCGGGCGCACCAAGGC
GTATTATCTGCGCGAAATCATCAATCATGGCCTGACTGACCTTGAGGATTACTACCTCGCGGCTGAAGTCCTGGAGCGTG
TCCGCAAAAATACAGAGGACGTTCACTCTGCTGCGGAGGTAAGGAAAGACCTTGGCCTGGACGATTGA