Detailed information of TA system TA047221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2251983..2252568
• Replicon: chromosome
• Accession: NZ_CP008696
• Organism: Pseudomonas chlororaphis strain PA23

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EY04_RS10070
• Protein ID: WP_038631367.1
• Coordinates: 2252275..2252568 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A554PQS3
• Locus tag: EY04_RS10065
• Protein ID: WP_038631365.1
• Coordinates: 2251983..2252273 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EY04_RS10055	2249495..2250877	+	1383	WP_038631363.1	efflux transporter outer membrane subunit	-
EY04_RS10060	2250996..2251583	+	588	WP_009048176.1	hypothetical protein	-
EY04_RS10065	2251983..2252273	-	291	WP_038631365.1	putative addiction module antidote protein	Antitoxin
EY04_RS10070	2252275..2252568	-	294	WP_038631367.1	addiction module antitoxin RelB	Toxin
EY04_RS10075	2252803..2254197	-	1395	WP_038631369.1	VOC family protein	-
EY04_RS10080	2254459..2255583	+	1125	WP_009048180.1	diguanylate cyclase	-
EY04_RS10085	2255677..2256921	+	1245	WP_038631373.1	M20/M25/M40 family metallo-hydrolase	-
EY04_RS10090	2257168..2257566	+	399	WP_016702749.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-97)

Antitoxin

• Uncharacterized protein HI1420 (8-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10757.31 Da
• Isoelectric Point: 9.6880

>T47221 WP_038631367.1 NZ_CP008696:c2252568-2252275 [Pseudomonas chlororaphis]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLDGGLSEMRVNTGAGYRLYFTVRGNTLIVLLLGGDK
SSQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T47221 NZ_CP008696:c2252568-2252275 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACACTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCGTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGACGGCGGTCTCTCGGAAATGCGCG
TGAATACAGGCGCCGGCTACCGCCTCTATTTCACGGTTCGCGGCAATACCCTCATCGTCCTGCTGCTGGGTGGCGACAAG
TCCAGCCAGCCCGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10249.81 Da
• Isoelectric Point: 8.0096

>AT47221 WP_038631365.1 NZ_CP008696:c2252273-2251983 [Pseudomonas chlororaphis]
MSDTLVPFDIAALLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKTTGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVPRG

Nucleotide

• Length: 291 bp

>AT47221 NZ_CP008696:c2252273-2251983 [Pseudomonas chlororaphis]
ATGTCCGACACACTGGTTCCCTTCGACATCGCCGCCCTGCTCGACAGCGACCAGGCGATCAGCGAATACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGACGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCCCGGGGCATGACGCAGATTGCCA
AGACCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGGGCCAAGCCGCGCTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGCCCCGAGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A554PQS3