Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 190978..191591 Replicon chromosome
Accession NZ_CP008696
Organism Pseudomonas chlororaphis strain PA23

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag EY04_RS00740 Protein ID WP_009041685.1
Coordinates 190978..191160 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag EY04_RS00745 Protein ID WP_009046450.1
Coordinates 191190..191591 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EY04_RS00715 186159..188123 + 1965 WP_164485207.1 choline transporter BetT -
EY04_RS00720 188239..188496 - 258 WP_009046444.1 hypothetical protein -
EY04_RS00725 188549..189037 - 489 WP_016702554.1 DoxX family protein -
EY04_RS00730 189094..189834 - 741 WP_009046448.1 SDR family oxidoreductase -
EY04_RS00735 189963..190853 + 891 WP_038630364.1 LysR family transcriptional regulator -
EY04_RS00740 190978..191160 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
EY04_RS00745 191190..191591 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EY04_RS00750 192160..193197 + 1038 WP_016702559.1 L-glyceraldehyde 3-phosphate reductase -
EY04_RS00755 193327..193755 - 429 WP_023970008.1 type II toxin-antitoxin system VapC family toxin -
EY04_RS00760 193752..193997 - 246 WP_016702561.1 plasmid stabilization protein -
EY04_RS00765 194137..194976 - 840 WP_009046454.1 taurine dioxygenase -
EY04_RS00770 195203..196042 - 840 WP_038630365.1 taurine ABC transporter permease TauC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T47218 WP_009041685.1 NZ_CP008696:190978-191160 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T47218 NZ_CP008696:190978-191160 [Pseudomonas chlororaphis]
ATGCGCAGTCGGGAACTGATCAGGATGATCGAGGAGGACGGGTGGTATCTGATCGCGGTCAAAGGCAGTCATCACCAATA
CAAGCATCTGCACAAGCCGGGACGGGTCACTATCCCTCACCCGGATGCGGATCTGCCCAGGGGCACGATCCACAGCATCT
TGAAACAGGCGGGCCTGAAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT47218 WP_009046450.1 NZ_CP008696:191190-191591 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT47218 NZ_CP008696:191190-191591 [Pseudomonas chlororaphis]
ATGAAATTCCCGGTCGTGCTGCACAAGGATGCCGACTCCGGATATGGAGTGATCGTCCCCGATGTGCCGGGCTGTTTTTC
CGCCGGTCATACCGCTGCGCAAGCCTTCGAGAATGTGAAAGAGGCCCTTTCGCTGCATTATGAAGGCCTGGTCGCCGATG
GTGAGCCGCTGCCCCAGGTGCATGAGGTCGACGTGCATATCGACAATCCTGATTACGCCGGTGGCGTGTGGGGCGTGATC
GAGTTCGACATCACGCCTTACTTCGGCAAGGCGGTGCGTTTCAATGCGACGTTGCCGGAGCAACTGTTGGAGCGCATCGA
CCAGACGGTGAAGCGCGACCAGCGCTACCGTTCGCGCTCGGGCTTTCTCGCGGCAGCGGCATTGCGCGAACTCTCCGCCT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References