Detailed information of TA system TA047203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3121133..3121737
• Replicon: chromosome
• Accession: NZ_CP007809
• Organism: Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: ER17_RS14870
• Protein ID: WP_003414492.1
• Coordinates: 3121133..3121525 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: ER17_RS14875
• Protein ID: WP_003414495.1
• Coordinates: 3121522..3121737 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER17_RS14840	3116283..3117071	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
ER17_RS14845	3117405..3117950	-	546	WP_014584866.1	DUF1802 family protein	-
ER17_RS14850	3118222..3119106	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
ER17_RS14855	3119109..3119996	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
ER17_RS14860	3120301..3120846	-	546	WP_003899500.1	DUF1802 family protein	-
ER17_RS14865	3120843..3121112	-	270	WP_003414489.1	DUF2277 family protein	-
ER17_RS14870	3121133..3121525	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
ER17_RS14875	3121522..3121737	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ER17_RS14880	3121784..3122533	+	750	WP_003902358.1	enoyl-CoA hydratase	-
ER17_RS14885	3122612..3123694	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
ER17_RS14890	3123687..3124997	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
ER17_RS14895	3125000..3125827	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
ER17_RS14900	3125824..3126735	-	912	WP_003902360.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T47203 WP_003414492.1 NZ_CP007809:c3121525-3121133 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T47203 NZ_CP007809:c3121525-3121133 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT47203 WP_003414495.1 NZ_CP007809:c3121737-3121522 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT47203 NZ_CP007809:c3121737-3121522 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8