47199

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3060938..3061617 Replicon chromosome
Accession NZ_CP007809
Organism Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag ER17_RS14520 Protein ID WP_003414059.1
Coordinates 3060938..3061354 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag ER17_RS14525 Protein ID WP_003414061.1
Coordinates 3061351..3061617 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER17_RS14500 3056990..3057892 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
ER17_RS14505 3057961..3058713 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
ER17_RS14510 3058957..3059232 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
ER17_RS14515 3059229..3060851 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
ER17_RS14520 3060938..3061354 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
ER17_RS14525 3061351..3061617 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
ER17_RS14530 3061643..3062038 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
ER17_RS14535 3062035..3062304 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
ER17_RS14540 3062314..3063408 - 1095 WP_003414068.1 restriction endonuclease subunit S -
ER17_RS14545 3063405..3063824 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
ER17_RS14550 3063898..3064377 - 480 WP_003414073.1 dihydrofolate reductase -
ER17_RS14555 3064448..3065248 - 801 WP_003911953.1 thymidylate synthase -
ER17_RS14560 3065404..3066141 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T47199 WP_003414059.1 NZ_CP007809:c3061354-3060938 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T47199 NZ_CP007809:c3061354-3060938 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT47199 WP_003414061.1 NZ_CP007809:c3061617-3061351 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT47199 NZ_CP007809:c3061617-3061351 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References