47196

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2861679..2862316 Replicon chromosome
Accession NZ_CP007809
Organism Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag ER17_RS13455 Protein ID WP_003413180.1
Coordinates 2861679..2862074 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag ER17_RS13460 Protein ID WP_003413183.1
Coordinates 2862071..2862316 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER17_RS13420 2857105..2857419 + 315 WP_009937839.1 hypothetical protein -
ER17_RS13425 2857715..2858926 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
ER17_RS13430 2859053..2859739 + 687 WP_038436266.1 hypothetical protein -
ER17_RS22340 2859736..2860013 + 278 Protein_2632 type II toxin-antitoxin system VapB family antitoxin -
ER17_RS13435 2860106..2860519 + 414 WP_003413164.1 PIN domain nuclease -
ER17_RS13440 2860558..2860815 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
ER17_RS13445 2860812..2861189 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
ER17_RS13450 2861205..2861579 - 375 WP_003413177.1 hypothetical protein -
ER17_RS13455 2861679..2862074 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
ER17_RS13460 2862071..2862316 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
ER17_RS13465 2862727..2863146 - 420 WP_003413190.1 A24 family peptidase -
ER17_RS13470 2863158..2863967 - 810 WP_003413193.1 shikimate dehydrogenase -
ER17_RS13475 2863964..2865217 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
ER17_RS13480 2865210..2865722 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T47196 WP_003413180.1 NZ_CP007809:c2862074-2861679 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T47196 NZ_CP007809:c2862074-2861679 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT47196 WP_003413183.1 NZ_CP007809:c2862316-2862071 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT47196 NZ_CP007809:c2862316-2862071 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References