Detailed information of TA system TA047193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2846852..2847492
• Replicon: chromosome
• Accession: NZ_CP007809
• Organism: Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: ER17_RS13360
• Protein ID: WP_003412970.1
• Coordinates: 2846852..2847271 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: ER17_RS13365
• Protein ID: WP_003412975.1
• Coordinates: 2847268..2847492 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER17_RS13335	2842442..2843164	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
ER17_RS13340	2843681..2843903	+	223	Protein_2612	type II toxin-antitoxin system VapB family antitoxin	-
ER17_RS13345	2843900..2844301	+	402	WP_003412963.1	PIN domain-containing protein	-
ER17_RS13350	2844336..2845256	-	921	WP_003902293.1	restriction endonuclease	-
ER17_RS22710	2845597..2845842	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ER17_RS13355	2845901..2846851	+	951	WP_003911916.1	Lsr2 family protein	-
ER17_RS13360	2846852..2847271	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
ER17_RS13365	2847268..2847492	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
ER17_RS13370	2847523..2850366	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
ER17_RS13375	2850438..2850839	-	402	WP_003412981.1	hypothetical protein	-
ER17_RS13380	2850839..2851309	-	471	WP_003899364.1	transcription antitermination factor NusB	-
ER17_RS13385	2851312..2851875	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T47193 WP_003412970.1 NZ_CP007809:c2847271-2846852 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T47193 NZ_CP007809:c2847271-2846852 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT47193 WP_003412975.1 NZ_CP007809:c2847492-2847268 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT47193 NZ_CP007809:c2847492-2847268 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09