Detailed information of TA system TA047182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2173718..2174421
• Replicon: chromosome
• Accession: NZ_CP007809
• Organism: Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: ER17_RS10090
• Protein ID: WP_003409778.1
• Coordinates: 2173718..2174047 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: ER17_RS21375
• Protein ID: WP_003409780.1
• Coordinates: 2174044..2174421 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER17_RS10070	2170101..2171171	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
ER17_RS10075	2171168..2171683	+	516	WP_003409718.1	flavin reductase family protein	-
ER17_RS10080	2171680..2172741	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
ER17_RS10085	2172738..2173508	+	771	WP_003409775.1	SDR family oxidoreductase	-
ER17_RS10090	2173718..2174047	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ER17_RS21375	2174044..2174421	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
ER17_RS10100	2174418..2175008	-	591	WP_003409784.1	SEC-C domain-containing protein	-
ER17_RS10105	2175063..2176427	+	1365	WP_003903691.1	HNH endonuclease	-
ER17_RS10110	2176582..2177034	-	453	WP_003899095.1	lipoprotein	-
ER17_RS10115	2177098..2177499	+	402	WP_003409869.1	hypothetical protein	-
ER17_RS10120	2177492..2177674	-	183	WP_019250140.1	hypothetical protein	-
ER17_RS10125	2177788..2178138	-	351	WP_003409871.1	hypothetical protein	-
ER17_RS10130	2178149..2179051	-	903	WP_003899097.1	hypothetical protein	-
ER17_RS10135	2179072..2179263	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T47182 WP_003409778.1 NZ_CP007809:c2174047-2173718 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T47182 NZ_CP007809:c2174047-2173718 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT47182 WP_003409780.1 NZ_CP007809:c2174421-2174044 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT47182 NZ_CP007809:c2174421-2174044 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8