Detailed information of TA system TA047168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 833421..834130
• Replicon: chromosome
• Accession: NZ_CP007809
• Organism: Mycobacterium tuberculosis strain KIT87190

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF74
• Locus tag: ER17_RS03915
• Protein ID: WP_003403837.1
• Coordinates: 833702..834130 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TEX4
• Locus tag: ER17_RS03910
• Protein ID: WP_003403834.1
• Coordinates: 833421..833678 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER17_RS03875	829041..829364	+	324	Protein_767	transposase	-
ER17_RS03880	829497..830024	+	528	WP_003901009.1	PE family protein	-
ER17_RS03885	830402..830959	-	558	WP_003403820.1	hypothetical protein	-
ER17_RS03890	830956..831462	-	507	WP_003403827.1	helix-turn-helix domain-containing protein	-
ER17_RS03895	831575..831721	-	147	WP_003403828.1	hypothetical protein	-
ER17_RS22930	831730..831974	+	245	Protein_772	DUF1998 domain-containing protein	-
ER17_RS03905	832217..833330	+	1114	Protein_773	PE family protein	-
ER17_RS03910	833421..833678	+	258	WP_003403834.1	ribbon-helix-helix protein, CopG family	Antitoxin
ER17_RS03915	833702..834130	+	429	WP_003403837.1	type II toxin-antitoxin system VapC family toxin	Toxin
ER17_RS03920	834220..834394	-	175	Protein_776	transposase	-
ER17_RS03925	834507..834752	+	246	WP_003403841.1	hypothetical protein	-
ER17_RS03930	834821..835705	-	885	WP_003403844.1	3-hydroxyisobutyrate dehydrogenase	-
ER17_RS03935	835716..836888	-	1173	WP_003901911.1	acyl-CoA dehydrogenase family protein	-
ER17_RS03940	836895..838427	-	1533	WP_003403847.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	829050..829364	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15831.27 Da
• Isoelectric Point: 7.5536

>T47168 WP_003403837.1 NZ_CP007809:833702-834130 [Mycobacterium tuberculosis]
MFLLDANVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLATNRRIFEIPSPRAEAFAFVEAVTAQPHHL
PTNPGPRHLMLLRKLCDEADASGDLIPDAVLAAIAVGHHCAVVSLDRDFARFASVRHIRPPL

Nucleotide

• Length: 429 bp

>T47168 NZ_CP007809:833702-834130 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9114.28 Da
• Isoelectric Point: 10.6530

>AT47168 WP_003403834.1 NZ_CP007809:833421-833678 [Mycobacterium tuberculosis]
MRTTVSISDEILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRALSEVLDEGLE
LNSRK

Nucleotide

• Length: 258 bp

>AT47168 NZ_CP007809:833421-833678 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829CDR3

Antitoxin

• AlphaFold DB: G0TEX4