Detailed information of TA system TA047150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 409940..410572
• Replicon: chromosome
• Accession: NZ_CP007806
• Organism: Brevibacillus laterosporus LMG 15441

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7EEZ8
• Locus tag: BRLA_RS02115
• Protein ID: WP_003333534.1
• Coordinates: 410222..410572 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BRLA_RS02110
• Protein ID: WP_173645602.1
• Coordinates: 409940..410218 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRLA_RS02085	405672..406004	+	333	WP_003333541.1	metalloregulator ArsR/SmtB family transcription factor	-
BRLA_RS02090	406215..406541	+	327	WP_003333540.1	hypothetical protein	-
BRLA_RS02095	406863..407894	+	1032	WP_003333538.1	DUF4367 domain-containing protein	-
BRLA_RS02100	408122..408565	+	444	WP_003333537.1	hypothetical protein	-
BRLA_RS02105	408569..409774	+	1206	WP_003333536.1	alanine racemase	-
BRLA_RS02110	409940..410218	+	279	WP_173645602.1	ribbon-helix-helix protein, CopG family	Antitoxin
BRLA_RS02115	410222..410572	+	351	WP_003333534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BRLA_RS02120	410802..412961	+	2160	WP_003333533.1	RNA-binding transcriptional accessory protein	-
BRLA_RS23540	413033..413158	-	126	WP_003333532.1	cortex morphogenetic protein CmpA	-
BRLA_RS02125	413270..413605	-	336	WP_031411755.1	hypothetical protein	-
BRLA_RS02130	413724..414188	+	465	WP_003333530.1	SprT family protein	-
BRLA_RS02135	414254..414724	+	471	WP_003333528.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
BRLA_RS02140	414705..415418	+	714	WP_003333527.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.83 Da
• Isoelectric Point: 4.8553

>T47150 WP_003333534.1 NZ_CP007806:410222-410572 [Brevibacillus laterosporus LMG 15441]
VNVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T47150 NZ_CP007806:410222-410572 [Brevibacillus laterosporus LMG 15441]
GTGAATGTTAAACGTGGCGATGTATTTTTCGCGGACCTTTCCCCTGTGGTTGGCTCCGAGCAAGGTGGAGTTAGACCGGT
ATTAGTTATACAAAACGATATTGGAAATCGATTTAGTCCGACGGTTATCGTCGCTGCGATCACTGCTCAGATTCAAAAGG
CAAAGCTGCCTACGCATGTGGAAATCGATGCAAAAACATATGGGTTTGATCGTGACTCTGTCATCTTATTGGAACAAATA
AGAACCATTGATAAGCAAAGGTTGACTGATAAAATCACTCATCTAGACGATGAGATGATGGAAAGAGTGAATGAATCACT
TCAGATCAGCCTCGGATTAATAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.43 Da
• Isoelectric Point: 9.6543

>AT47150 WP_173645602.1 NZ_CP007806:409940-410218 [Brevibacillus laterosporus LMG 15441]
MSKSNTKRIMISLPQHLLQEVDGVVQKERSNRSELIRQAMSLYLKERKKRFIRETMQRGYLEMAKINLNMASEAFEAEEE
ADLTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT47150 NZ_CP007806:409940-410218 [Brevibacillus laterosporus LMG 15441]
TTGTCTAAAAGCAATACAAAACGTATCATGATCAGTTTGCCGCAACATTTATTGCAAGAGGTAGACGGCGTCGTGCAGAA
AGAGAGATCAAACCGAAGTGAATTGATTCGTCAAGCGATGAGTCTCTACCTCAAGGAACGAAAAAAACGCTTTATACGAG
AAACAATGCAACGTGGCTATTTGGAAATGGCTAAAATCAATCTCAATATGGCATCGGAGGCTTTTGAAGCAGAAGAGGAA
GCCGATCTTACCTTAGACCGCTTAGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0F7EEZ8