Detailed information of TA system TA047142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3748827..3749356
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: MTBK_RS17600
• Protein ID: WP_003417760.1
• Coordinates: 3749099..3749356 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: MTBK_RS17595
• Protein ID: WP_003417757.1
• Coordinates: 3748827..3749102 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS17565	3745400..3746194	-	795	WP_003417738.1	BBE domain-containing protein	-
MTBK_RS17570	3746276..3746647	-	372	WP_003417739.1	FAD-binding oxidoreductase	-
MTBK_RS22370	3746545..3746763	-	219	WP_157132559.1	hypothetical protein	-
MTBK_RS17575	3746790..3747050	-	261	WP_003417741.1	hypothetical protein	-
MTBK_RS17580	3747165..3747554	+	390	WP_003417745.1	DUF732 domain-containing protein	-
MTBK_RS17585	3747568..3747861	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
MTBK_RS17590	3747858..3748703	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
MTBK_RS17595	3748827..3749102	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
MTBK_RS17600	3749099..3749356	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
MTBK_RS17605	3749398..3750588	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
MTBK_RS17610	3750705..3751073	+	369	WP_003417765.1	FHA domain-containing protein	-
MTBK_RS17615	3751070..3751621	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
MTBK_RS17620	3751628..3752209	-	582	WP_003417769.1	ATP/GTP-binding protein	-
MTBK_RS17625	3752190..3752558	-	369	WP_003417772.1	DUF742 domain-containing protein	-
MTBK_RS17630	3752536..3752928	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T47142 WP_003417760.1 NZ_CP007803:3749099-3749356 [Mycobacterium tuberculosis K]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T47142 NZ_CP007803:3749099-3749356 [Mycobacterium tuberculosis K]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT47142 WP_003417757.1 NZ_CP007803:3748827-3749102 [Mycobacterium tuberculosis K]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT47142 NZ_CP007803:3748827-3749102 [Mycobacterium tuberculosis K]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94