47139

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3158135..3158822 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag MTBK_RS15025 Protein ID WP_003414624.1
Coordinates 3158379..3158822 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag MTBK_RS15020 Protein ID WP_003414620.1
Coordinates 3158135..3158392 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS15000 3153455..3154309 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
MTBK_RS15005 3154365..3155528 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
MTBK_RS15010 3155545..3156759 - 1215 WP_003414610.1 zinc metalloprotease Rip -
MTBK_RS15015 3156767..3158008 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
MTBK_RS15020 3158135..3158392 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
MTBK_RS15025 3158379..3158822 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
MTBK_RS15030 3158902..3159564 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
MTBK_RS15035 3159660..3159851 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MTBK_RS15040 3159844..3161931 + 2088 WP_003414635.1 cytochrome c biogenesis protein DipZ -
MTBK_RS15045 3162027..3162608 + 582 WP_003414644.1 fasciclin domain-containing protein -
MTBK_RS15050 3162660..3162974 + 315 WP_003899522.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T47139 WP_003414624.1 NZ_CP007803:3158379-3158822 [Mycobacterium tuberculosis K]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T47139 NZ_CP007803:3158379-3158822 [Mycobacterium tuberculosis K]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT47139 WP_003414620.1 NZ_CP007803:3158135-3158392 [Mycobacterium tuberculosis K]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT47139 NZ_CP007803:3158135-3158392 [Mycobacterium tuberculosis K]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
AlphaFold DB G0TFH0

References