Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3091526..3092096 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag MTBK_RS14665 Protein ID WP_003414166.1
Coordinates 3091526..3091882 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag MTBK_RS14670 Protein ID WP_003901465.1
Coordinates 3091866..3092096 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS14645 3086978..3088666 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
MTBK_RS14650 3088670..3088996 - 327 WP_003414157.1 hypothetical protein -
MTBK_RS14655 3089127..3089756 + 630 WP_003414158.1 DUF3558 family protein -
MTBK_RS14660 3089775..3091424 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
MTBK_RS14665 3091526..3091882 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
MTBK_RS14670 3091866..3092096 - 231 WP_003901465.1 antitoxin MazE Antitoxin
MTBK_RS14675 3092139..3093182 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
MTBK_RS22135 3093373..3093648 + 276 WP_003911993.1 DUF1778 domain-containing protein -
MTBK_RS22365 3093824..3094078 - 255 WP_003917684.1 hypothetical protein -
MTBK_RS14690 3094226..3094630 + 405 WP_003414181.1 hypothetical protein -
MTBK_RS14695 3094627..3094818 + 192 WP_003414184.1 hypothetical protein -
MTBK_RS14700 3095017..3096171 + 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
MTBK_RS14705 3096405..3096662 + 258 WP_003899489.1 hypothetical protein -
MTBK_RS14710 3096767..3097078 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T47135 WP_003414166.1 NZ_CP007803:c3091882-3091526 [Mycobacterium tuberculosis K]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T47135 NZ_CP007803:c3091882-3091526 [Mycobacterium tuberculosis K]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT47135 WP_003901465.1 NZ_CP007803:c3092096-3091866 [Mycobacterium tuberculosis K]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT47135 NZ_CP007803:c3092096-3091866 [Mycobacterium tuberculosis K]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References