Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3051942..3052629 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag MTBK_RS14455 Protein ID WP_003414064.1
Coordinates 3052234..3052629 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag MTBK_RS14450 Protein ID WP_003414061.1
Coordinates 3051942..3052208 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS14425 3047581..3048483 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
MTBK_RS14430 3048552..3049304 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
MTBK_RS14435 3049548..3049823 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
MTBK_RS14440 3049820..3051442 - 1623 WP_003414057.1 type I restriction-modification system subunit M -
MTBK_RS14445 3051529..3051945 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
MTBK_RS14450 3051942..3052208 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MTBK_RS14455 3052234..3052629 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
MTBK_RS14460 3052626..3052895 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
MTBK_RS14465 3052905..3053999 - 1095 WP_038437371.1 restriction endonuclease subunit S -
MTBK_RS14470 3053996..3054415 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
MTBK_RS14475 3054489..3054968 - 480 WP_003414073.1 dihydrofolate reductase -
MTBK_RS14480 3055039..3055839 - 801 WP_003911953.1 thymidylate synthase -
MTBK_RS14485 3055995..3056732 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T47134 WP_003414064.1 NZ_CP007803:c3052629-3052234 [Mycobacterium tuberculosis K]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T47134 NZ_CP007803:c3052629-3052234 [Mycobacterium tuberculosis K]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT47134 WP_003414061.1 NZ_CP007803:c3052208-3051942 [Mycobacterium tuberculosis K]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT47134 NZ_CP007803:c3052208-3051942 [Mycobacterium tuberculosis K]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References