Detailed information of TA system TA047133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3051529..3052208
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: MTBK_RS14445
• Protein ID: WP_003414059.1
• Coordinates: 3051529..3051945 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: MTBK_RS14450
• Protein ID: WP_003414061.1
• Coordinates: 3051942..3052208 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS14425	3047581..3048483	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
MTBK_RS14430	3048552..3049304	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
MTBK_RS14435	3049548..3049823	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
MTBK_RS14440	3049820..3051442	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
MTBK_RS14445	3051529..3051945	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
MTBK_RS14450	3051942..3052208	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MTBK_RS14455	3052234..3052629	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
MTBK_RS14460	3052626..3052895	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
MTBK_RS14465	3052905..3053999	-	1095	WP_038437371.1	restriction endonuclease subunit S	-
MTBK_RS14470	3053996..3054415	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
MTBK_RS14475	3054489..3054968	-	480	WP_003414073.1	dihydrofolate reductase	-
MTBK_RS14480	3055039..3055839	-	801	WP_003911953.1	thymidylate synthase	-
MTBK_RS14485	3055995..3056732	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T47133 WP_003414059.1 NZ_CP007803:c3051945-3051529 [Mycobacterium tuberculosis K]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T47133 NZ_CP007803:c3051945-3051529 [Mycobacterium tuberculosis K]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT47133 WP_003414061.1 NZ_CP007803:c3052208-3051942 [Mycobacterium tuberculosis K]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT47133 NZ_CP007803:c3052208-3051942 [Mycobacterium tuberculosis K]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64