Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2852341..2852978 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag MTBK_RS13380 Protein ID WP_003413180.1
Coordinates 2852341..2852736 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag MTBK_RS13385 Protein ID WP_003413183.1
Coordinates 2852733..2852978 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS13345 2847745..2848956 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
MTBK_RS13350 2849083..2849742 + 660 WP_003902296.1 hypothetical protein -
MTBK_RS13355 2849739..2850401 + 663 WP_003905878.1 hypothetical protein -
MTBK_RS22120 2850398..2850675 + 278 Protein_2631 type II toxin-antitoxin system VapB family antitoxin -
MTBK_RS13360 2850768..2851181 + 414 WP_003413164.1 PIN domain nuclease -
MTBK_RS13365 2851220..2851477 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
MTBK_RS13370 2851474..2851851 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
MTBK_RS13375 2851867..2852241 - 375 WP_003413177.1 hypothetical protein -
MTBK_RS13380 2852341..2852736 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
MTBK_RS13385 2852733..2852978 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
MTBK_RS13390 2853389..2853808 - 420 WP_003413190.1 A24 family peptidase -
MTBK_RS13395 2853820..2854629 - 810 WP_003413193.1 shikimate dehydrogenase -
MTBK_RS13400 2854626..2855879 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
MTBK_RS13405 2855872..2856384 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T47130 WP_003413180.1 NZ_CP007803:c2852736-2852341 [Mycobacterium tuberculosis K]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T47130 NZ_CP007803:c2852736-2852341 [Mycobacterium tuberculosis K]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT47130 WP_003413183.1 NZ_CP007803:c2852978-2852733 [Mycobacterium tuberculosis K]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT47130 NZ_CP007803:c2852978-2852733 [Mycobacterium tuberculosis K]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References