Detailed information of TA system TA047129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2851220..2851851
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: MTBK_RS13370
• Protein ID: WP_003413174.1
• Coordinates: 2851474..2851851 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: MTBK_RS13365
• Protein ID: WP_003413167.1
• Coordinates: 2851220..2851477 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS13340	2847042..2847449	+	408	WP_003413029.1	hypothetical protein	-
MTBK_RS13345	2847745..2848956	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
MTBK_RS13350	2849083..2849742	+	660	WP_003902296.1	hypothetical protein	-
MTBK_RS13355	2849739..2850401	+	663	WP_003905878.1	hypothetical protein	-
MTBK_RS22120	2850398..2850675	+	278	Protein_2631	type II toxin-antitoxin system VapB family antitoxin	-
MTBK_RS13360	2850768..2851181	+	414	WP_003413164.1	PIN domain nuclease	-
MTBK_RS13365	2851220..2851477	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
MTBK_RS13370	2851474..2851851	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTBK_RS13375	2851867..2852241	-	375	WP_003413177.1	hypothetical protein	-
MTBK_RS13380	2852341..2852736	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
MTBK_RS13385	2852733..2852978	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
MTBK_RS13390	2853389..2853808	-	420	WP_003413190.1	A24 family peptidase	-
MTBK_RS13395	2853820..2854629	-	810	WP_003413193.1	shikimate dehydrogenase	-
MTBK_RS13400	2854626..2855879	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
MTBK_RS13405	2855872..2856384	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T47129 WP_003413174.1 NZ_CP007803:2851474-2851851 [Mycobacterium tuberculosis K]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T47129 NZ_CP007803:2851474-2851851 [Mycobacterium tuberculosis K]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT47129 WP_003413167.1 NZ_CP007803:2851220-2851477 [Mycobacterium tuberculosis K]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT47129 NZ_CP007803:2851220-2851477 [Mycobacterium tuberculosis K]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5