Detailed information of TA system TA047127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2836882..2837522
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: MTBK_RS13280
• Protein ID: WP_003412970.1
• Coordinates: 2836882..2837301 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: MTBK_RS13285
• Protein ID: WP_003412975.1
• Coordinates: 2837298..2837522 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS13255	2832472..2833194	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
MTBK_RS13260	2833711..2833933	+	223	Protein_2610	type II toxin-antitoxin system VapB family antitoxin	-
MTBK_RS13265	2833930..2834331	+	402	WP_003412963.1	PIN domain-containing protein	-
MTBK_RS13270	2834366..2835286	-	921	WP_003902293.1	restriction endonuclease	-
MTBK_RS22445	2835627..2835872	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
MTBK_RS13275	2835931..2836881	+	951	WP_003911916.1	Lsr2 family protein	-
MTBK_RS13280	2836882..2837301	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTBK_RS13285	2837298..2837522	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
MTBK_RS13290	2837553..2840396	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
MTBK_RS13295	2840468..2840869	-	402	WP_003412981.1	hypothetical protein	-
MTBK_RS13300	2840869..2841339	-	471	WP_003899364.1	transcription antitermination factor NusB	-
MTBK_RS13305	2841342..2841905	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T47127 WP_003412970.1 NZ_CP007803:c2837301-2836882 [Mycobacterium tuberculosis K]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T47127 NZ_CP007803:c2837301-2836882 [Mycobacterium tuberculosis K]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT47127 WP_003412975.1 NZ_CP007803:c2837522-2837298 [Mycobacterium tuberculosis K]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT47127 NZ_CP007803:c2837522-2837298 [Mycobacterium tuberculosis K]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09