47121

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2235069..2235710 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag MTBK_RS10440 Protein ID WP_003410075.1
Coordinates 2235312..2235710 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLM7
Locus tag MTBK_RS10435 Protein ID WP_003899127.1
Coordinates 2235069..2235311 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS10425 2233123..2233467 - 345 WP_003410065.1 ferredoxin family protein -
MTBK_RS10430 2233656..2234981 - 1326 WP_003410070.1 DUF4143 domain-containing protein -
MTBK_RS10435 2235069..2235311 + 243 WP_003899127.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MTBK_RS10440 2235312..2235710 + 399 WP_003410075.1 PIN domain nuclease Toxin
MTBK_RS10445 2235893..2236324 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
MTBK_RS10450 2236365..2236859 + 495 WP_003410080.1 DUF1398 domain-containing protein -
MTBK_RS21235 2237296..2237475 - 180 Protein_2065 hypothetical protein -
MTBK_RS10455 2237536..2238183 + 648 WP_071854215.1 transposase -
MTBK_RS10460 2238137..2238727 + 591 WP_003899131.1 IS110 family transposase -
MTBK_RS10465 2238855..2240111 - 1257 WP_003902247.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(7-48)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T47121 WP_003410075.1 NZ_CP007803:2235312-2235710 [Mycobacterium tuberculosis K]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T47121 NZ_CP007803:2235312-2235710 [Mycobacterium tuberculosis K]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8851.73 Da        Isoelectric Point: 4.1621

>AT47121 WP_003899127.1 NZ_CP007803:2235069-2235311 [Mycobacterium tuberculosis K]
VYSGVVSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 243 bp

>AT47121 NZ_CP007803:2235069-2235311 [Mycobacterium tuberculosis K]
GTGTATAGTGGTGTTGTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACT
CGATTCCAAGCGAAGTGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGC
TGCAGGGCAGCGGTTTCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A045JPF9

References