Detailed information of TA system TA047115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2167189..2167892
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: MTBK_RS10025
• Protein ID: WP_003409778.1
• Coordinates: 2167189..2167518 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: MTBK_RS21200
• Protein ID: WP_003409780.1
• Coordinates: 2167515..2167892 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS10005	2163572..2164642	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
MTBK_RS10010	2164639..2165154	+	516	WP_003409718.1	flavin reductase family protein	-
MTBK_RS10015	2165151..2166212	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MTBK_RS10020	2166209..2166979	+	771	WP_003409775.1	SDR family oxidoreductase	-
MTBK_RS10025	2167189..2167518	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MTBK_RS21200	2167515..2167892	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
MTBK_RS10035	2167889..2168479	-	591	WP_003409784.1	SEC-C domain-containing protein	-
MTBK_RS10040	2168534..2169898	+	1365	WP_003903691.1	HNH endonuclease	-
MTBK_RS10045	2170053..2170505	-	453	WP_003899095.1	lipoprotein	-
MTBK_RS10050	2170569..2170970	+	402	WP_003409869.1	hypothetical protein	-
MTBK_RS10055	2170963..2171145	-	183	WP_019250140.1	hypothetical protein	-
MTBK_RS10060	2171259..2171609	-	351	WP_003409871.1	hypothetical protein	-
MTBK_RS10065	2171620..2172522	-	903	WP_003899097.1	hypothetical protein	-
MTBK_RS10070	2172543..2172734	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T47115 WP_003409778.1 NZ_CP007803:c2167518-2167189 [Mycobacterium tuberculosis K]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T47115 NZ_CP007803:c2167518-2167189 [Mycobacterium tuberculosis K]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT47115 WP_003409780.1 NZ_CP007803:c2167892-2167515 [Mycobacterium tuberculosis K]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT47115 NZ_CP007803:c2167892-2167515 [Mycobacterium tuberculosis K]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8