Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1928243..1928856 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag MTBK_RS08940 Protein ID WP_003408465.1
Coordinates 1928243..1928632 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag MTBK_RS08945 Protein ID WP_003408469.1
Coordinates 1928629..1928856 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS08905 1923872..1924786 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
MTBK_RS08910 1924789..1925619 + 831 WP_003408448.1 cyclase family protein -
MTBK_RS08915 1925619..1925969 + 351 WP_003898986.1 cupin domain-containing protein -
MTBK_RS08920 1926022..1926840 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
MTBK_RS08925 1926854..1927633 + 780 WP_003408460.1 IclR family transcriptional regulator -
MTBK_RS08940 1928243..1928632 - 390 WP_003408465.1 PIN domain-containing protein Toxin
MTBK_RS08945 1928629..1928856 - 228 WP_003408469.1 antitoxin Antitoxin
MTBK_RS08950 1929074..1930558 + 1485 WP_003408473.1 carboxylase -
MTBK_RS08955 1930555..1931802 + 1248 WP_003408476.1 serine hydrolase -
MTBK_RS08960 1931845..1932264 - 420 WP_003408483.1 hypothetical protein -
MTBK_RS08965 1932254..1932964 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T47113 WP_003408465.1 NZ_CP007803:c1928632-1928243 [Mycobacterium tuberculosis K]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T47113 NZ_CP007803:c1928632-1928243 [Mycobacterium tuberculosis K]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT47113 WP_003408469.1 NZ_CP007803:c1928856-1928629 [Mycobacterium tuberculosis K]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT47113 NZ_CP007803:c1928856-1928629 [Mycobacterium tuberculosis K]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References