Detailed information of TA system TA047107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1229328..1229896
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: MTBK_RS05860
• Protein ID: WP_003405865.1
• Coordinates: 1229522..1229896 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: MTBK_RS05855
• Protein ID: WP_003405863.1
• Coordinates: 1229328..1229525 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS05835	1225369..1226007	-	639	WP_003898730.1	lipid droplet-associated protein	-
MTBK_RS05840	1226097..1227104	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
MTBK_RS05845	1227121..1228104	-	984	WP_003898731.1	hypothetical protein	-
MTBK_RS05850	1228167..1229240	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
MTBK_RS05855	1229328..1229525	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MTBK_RS05860	1229522..1229896	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
MTBK_RS05865	1230099..1230797	+	699	WP_003898733.1	hypothetical protein	-
MTBK_RS05870	1230915..1231100	+	186	WP_003901093.1	hypothetical protein	-
MTBK_RS05875	1231027..1231302	-	276	WP_003405867.1	hypothetical protein	-
MTBK_RS05880	1231545..1231868	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
MTBK_RS05885	1231883..1232743	-	861	WP_003898734.1	hypothetical protein	-
MTBK_RS05895	1232776..1233546	-	771	Protein_1160	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T47107 WP_003405865.1 NZ_CP007803:1229522-1229896 [Mycobacterium tuberculosis K]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T47107 NZ_CP007803:1229522-1229896 [Mycobacterium tuberculosis K]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT47107 WP_003405863.1 NZ_CP007803:1229328-1229525 [Mycobacterium tuberculosis K]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT47107 NZ_CP007803:1229328-1229525 [Mycobacterium tuberculosis K]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07