Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1220572..1221203 Replicon chromosome
Accession NZ_CP007803
Organism Mycobacterium tuberculosis K

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag MTBK_RS05800 Protein ID WP_003405820.1
Coordinates 1220572..1220883 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag MTBK_RS05805 Protein ID WP_003405836.1
Coordinates 1220883..1221203 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTBK_RS05780 1216053..1217477 - 1425 WP_003405805.1 class II fumarate hydratase -
MTBK_RS05785 1217508..1218596 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
MTBK_RS05790 1218595..1219296 + 702 WP_003405815.1 DUF4245 domain-containing protein -
MTBK_RS05795 1219303..1220460 - 1158 WP_003898727.1 AI-2E family transporter -
MTBK_RS05800 1220572..1220883 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
MTBK_RS05805 1220883..1221203 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
MTBK_RS20940 1221213..1222738 + 1526 Protein_1145 carboxylesterase/lipase family protein -
MTBK_RS05820 1222756..1223868 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
MTBK_RS05825 1223878..1224135 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
MTBK_RS05830 1224125..1225372 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
MTBK_RS05835 1225369..1226007 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T47106 WP_003405820.1 NZ_CP007803:c1220883-1220572 [Mycobacterium tuberculosis K]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T47106 NZ_CP007803:c1220883-1220572 [Mycobacterium tuberculosis K]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT47106 WP_003405836.1 NZ_CP007803:c1221203-1220883 [Mycobacterium tuberculosis K]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT47106 NZ_CP007803:c1221203-1220883 [Mycobacterium tuberculosis K]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References