Detailed information of TA system TA047088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 357955..358598
• Replicon: chromosome
• Accession: NZ_CP007803
• Organism: Mycobacterium tuberculosis K

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB8
• Locus tag: MTBK_RS01580
• Protein ID: WP_003401566.1
• Coordinates: 358173..358598 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: MTBK_RS01575
• Protein ID: WP_003401563.1
• Coordinates: 357955..358176 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTBK_RS01550	353074..353877	-	804	WP_003401540.1	hypothetical protein	-
MTBK_RS01555	353887..355284	-	1398	WP_003401544.1	sulfatase	-
MTBK_RS01560	355463..357238	+	1776	WP_031655214.1	PE family protein	-
MTBK_RS01565	357381..357608	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
MTBK_RS01570	357605..357907	+	303	WP_003401560.1	toxin-antitoxin system toxin	-
MTBK_RS01575	357955..358176	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
MTBK_RS01580	358173..358598	+	426	WP_003401566.1	PIN domain nuclease	Toxin
MTBK_RS01585	358723..359367	+	645	WP_038437319.1	TetR/AcrR family transcriptional regulator	-
MTBK_RS01590	359364..360272	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15713.90 Da
• Isoelectric Point: 5.6002

>T47088 WP_003401566.1 NZ_CP007803:358173-358598 [Mycobacterium tuberculosis K]
VTDQRWLIDKSALVRLTDSPDMEIWSNRIERGLVHITGVTRLEVGFSAECGEIARREFREPPLSAMPVEYLTPRIEDRAL
EVQTLLADRGHHRGPSIPDLLIAATAELSGLTVLHVDKDFDAIAALTGQKTERLTHRPPSA

Nucleotide

• Length: 426 bp

>T47088 NZ_CP007803:358173-358598 [Mycobacterium tuberculosis K]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT47088 WP_003401563.1 NZ_CP007803:357955-358176 [Mycobacterium tuberculosis K]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT47088 NZ_CP007803:357955-358176 [Mycobacterium tuberculosis K]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Toxin

• PDB: 3H87

Antitoxin

• AlphaFold DB: A0A829CG48