Detailed information of TA system TA047058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 502203..502839
• Replicon: chromosome
• Accession: NZ_CP007800
• Organism: Bacillus subtilis subsp. subtilis str. JH642 substr. AG174 strain JH642

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BSUA_RS02605
• Protein ID: WP_003156187.1
• Coordinates: 502489..502839 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: BSUA_RS02600
• Protein ID: WP_003225183.1
• Coordinates: 502203..502484 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSUA_RS02580	498562..499161	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
BSUA_RS02585	499256..499621	+	366	WP_003234281.1	holo-ACP synthase	-
BSUA_RS02590	499787..500803	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
BSUA_RS02595	500918..502087	+	1170	WP_003234284.1	alanine racemase	-
BSUA_RS02600	502203..502484	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BSUA_RS02605	502489..502839	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BSUA_RS02610	502954..503778	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
BSUA_RS02615	503783..504148	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
BSUA_RS02620	504152..504553	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
BSUA_RS02625	504565..505572	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
BSUA_RS02630	505634..505963	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
BSUA_RS02635	505960..506442	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
BSUA_RS02640	506408..507196	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
BSUA_RS02645	507196..507795	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T47058 WP_003156187.1 NZ_CP007800:502489-502839 [Bacillus subtilis subsp. subtilis str. JH642 substr. AG174]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T47058 NZ_CP007800:502489-502839 [Bacillus subtilis subsp. subtilis str. JH642 substr. AG174]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT47058 WP_003225183.1 NZ_CP007800:502203-502484 [Bacillus subtilis subsp. subtilis str. JH642 substr. AG174]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT47058 NZ_CP007800:502203-502484 [Bacillus subtilis subsp. subtilis str. JH642 substr. AG174]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32