Detailed information of TA system TA047053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5199553..5200069
• Replicon: chromosome
• Accession: NZ_CP007799
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECOLIN_RS25905
• Protein ID: WP_001554709.1
• Coordinates: 5199553..5199837 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8H9DQ25
• Locus tag: ECOLIN_RS25910
• Protein ID: WP_001554710.1
• Coordinates: 5199827..5200069 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECOLIN_RS25875	5195117..5195716	+	600	WP_077769348.1	replication initiation protein	-
ECOLIN_RS25880 (ECOLIN_25105)	5195706..5196353	+	648	WP_024199421.1	hypothetical protein	-
ECOLIN_RS25885 (ECOLIN_25110)	5196356..5197498	+	1143	WP_001704287.1	DUF4868 domain-containing protein	-
ECOLIN_RS25890 (ECOLIN_25115)	5197725..5198051	+	327	WP_001554615.1	MobC family plasmid mobilization relaxosome protein	-
ECOLIN_RS29935	5198041..5198832	+	792	Protein_4896	relaxase/mobilization nuclease domain-containing protein	-
ECOLIN_RS25900 (ECOLIN_25125)	5199275..5199508	+	234	WP_001554708.1	MbeD family mobilization/exclusion protein	-
ECOLIN_RS25905 (ECOLIN_25130)	5199553..5199837	-	285	WP_001554709.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECOLIN_RS25910 (ECOLIN_25135)	5199827..5200069	-	243	WP_001554710.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ECOLIN_RS25915 (ECOLIN_25140)	5200334..5201230	+	897	WP_024199422.1	replication initiation protein	-
ECOLIN_RS25920 (ECOLIN_25145)	5201220..5201867	+	648	WP_024199421.1	hypothetical protein	-
ECOLIN_RS32560	5202344..5202664	+	321	WP_193790566.1	nikA protein	-
ECOLIN_RS25935 (ECOLIN_25155)	5203053..5203481	-	429	WP_000222531.1	helix-turn-helix domain-containing protein	-
ECOLIN_RS32430	5203639..5203809	+	171	WP_001531327.1	hypothetical protein	-
ECOLIN_RS25940 (ECOLIN_25160)	5203773..5204201	+	429	WP_000534558.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-77)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11265.07 Da
• Isoelectric Point: 10.4971

>T47053 WP_001554709.1 NZ_CP007799:c5199837-5199553 [Escherichia coli Nissle 1917]
MSYNLEFDPRALKEWRKLGDDVRLQFKKKLEQVLQHPRIDKNRLRELHDCYKIKLRASGYRLVYQVRDQTITVFVVAVGK
RERSAAYDAADKRL

Nucleotide

• Length: 285 bp

>T47053 NZ_CP007799:c5199837-5199553 [Escherichia coli Nissle 1917]
ATGAGCTATAACCTTGAATTTGATCCCCGAGCCCTGAAGGAATGGCGCAAGCTCGGGGATGATGTCCGTCTGCAGTTCAA
GAAAAAACTCGAGCAGGTTCTACAACACCCGCGGATCGATAAAAATCGCCTGCGAGAGCTGCATGACTGCTACAAAATCA
AGCTCCGTGCATCCGGTTATCGCTTGGTCTATCAGGTTCGCGATCAAACCATTACGGTATTCGTGGTGGCGGTCGGTAAG
CGCGAGCGTTCTGCCGCTTACGATGCGGCCGATAAACGCTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8958.21 Da
• Isoelectric Point: 4.2821

>AT47053 WP_001554710.1 NZ_CP007799:c5200069-5199827 [Escherichia coli Nissle 1917]
MATINIRIDDELKSRSYAALEKLGVTPSEVLRQTLEYVAQSGRLPFQQVLLTEDDADLMAIVRDRLENPQAGVKVSLDEL

Nucleotide

• Length: 243 bp

>AT47053 NZ_CP007799:c5200069-5199827 [Escherichia coli Nissle 1917]
ATGGCCACGATTAATATTCGGATCGATGACGAGCTGAAAAGCCGCTCTTATGCCGCACTGGAAAAGCTGGGCGTAACGCC
GTCCGAGGTTCTGCGCCAAACACTGGAATATGTGGCCCAAAGCGGACGTTTGCCGTTCCAGCAGGTTTTGCTGACCGAGG
ATGATGCCGATTTGATGGCTATCGTTCGGGATCGTCTGGAAAACCCACAGGCGGGCGTAAAGGTGTCACTGGATGAGCTA
TAA