Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5024001..5024577 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag ECOLIN_RS24970 Protein ID WP_001295743.1
Coordinates 5024001..5024288 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag ECOLIN_RS24975 Protein ID WP_000063148.1
Coordinates 5024275..5024577 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS24955 (ECOLIN_24175) 5019661..5020965 - 1305 WP_000535012.1 restriction endonuclease subunit S -
ECOLIN_RS24960 (ECOLIN_24180) 5020955..5022574 - 1620 WP_001029745.1 type I restriction-modification system subunit M -
ECOLIN_RS24965 (ECOLIN_24185) 5022638..5023804 - 1167 WP_000800831.1 restriction endonuclease -
ECOLIN_RS24970 (ECOLIN_24190) 5024001..5024288 + 288 WP_001295743.1 BrnT family toxin Toxin
ECOLIN_RS24975 (ECOLIN_24195) 5024275..5024577 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
ECOLIN_RS24980 (ECOLIN_24200) 5024611..5025567 - 957 WP_001295745.1 GTPase -
ECOLIN_RS24985 (ECOLIN_24205) 5025578..5025781 - 204 WP_000467859.1 YbdD/YjiX family protein -
ECOLIN_RS24990 (ECOLIN_24210) 5025833..5027983 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T47052 WP_001295743.1 NZ_CP007799:5024001-5024288 [Escherichia coli Nissle 1917]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T47052 NZ_CP007799:5024001-5024288 [Escherichia coli Nissle 1917]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT47052 WP_000063148.1 NZ_CP007799:5024275-5024577 [Escherichia coli Nissle 1917]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT47052 NZ_CP007799:5024275-5024577 [Escherichia coli Nissle 1917]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References