Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4816660..4817157 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECOLIN_RS23890 Protein ID WP_001173343.1
Coordinates 4816984..4817157 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag ECOLIN_RS23885 Protein ID WP_001312331.1
Coordinates 4816660..4816956 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS23865 (ECOLIN_23120) 4813389..4814108 - 720 WP_000611291.1 two-component system response regulator DcuR -
ECOLIN_RS23870 (ECOLIN_23125) 4814105..4815736 - 1632 WP_001216466.1 sensor histidine kinase -
ECOLIN_RS23875 (ECOLIN_23130) 4815917..4816147 + 231 WP_000371715.1 (4Fe-4S)-binding protein -
ECOLIN_RS23880 (ECOLIN_23135) 4816159..4816431 + 273 WP_000405647.1 N-acetyltransferase -
ECOLIN_RS23885 (ECOLIN_23140) 4816660..4816956 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECOLIN_RS23890 4816984..4817157 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECOLIN_RS23895 (ECOLIN_23150) 4817276..4818793 - 1518 WP_000003806.1 lysine--tRNA ligase -
ECOLIN_RS23900 (ECOLIN_23155) 4819030..4820487 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T47050 WP_001173343.1 NZ_CP007799:4816984-4817157 [Escherichia coli Nissle 1917]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T47050 NZ_CP007799:4816984-4817157 [Escherichia coli Nissle 1917]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT47050 WP_001312331.1 NZ_CP007799:4816660-4816956 [Escherichia coli Nissle 1917]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT47050 NZ_CP007799:4816660-4816956 [Escherichia coli Nissle 1917]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References