Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3273020..3273674 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ECOLIN_RS16135 Protein ID WP_000244781.1
Coordinates 3273020..3273427 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECOLIN_RS16140 Protein ID WP_000354046.1
Coordinates 3273408..3273674 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS16115 (ECOLIN_15595) 3268977..3270710 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
ECOLIN_RS16120 (ECOLIN_15600) 3270716..3271426 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECOLIN_RS16125 (ECOLIN_15605) 3271451..3272347 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECOLIN_RS16130 (ECOLIN_15610) 3272459..3272980 + 522 WP_001055874.1 flavodoxin FldB -
ECOLIN_RS16135 (ECOLIN_15615) 3273020..3273427 - 408 WP_000244781.1 protein YgfX Toxin
ECOLIN_RS16140 (ECOLIN_15620) 3273408..3273674 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECOLIN_RS16145 (ECOLIN_15630) 3273917..3274897 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
ECOLIN_RS16150 (ECOLIN_15635) 3274974..3275633 - 660 WP_000250274.1 hemolysin III family protein -
ECOLIN_RS16155 (ECOLIN_15640) 3275797..3276108 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
ECOLIN_RS16160 (ECOLIN_15645) 3276153..3277586 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T47044 WP_000244781.1 NZ_CP007799:c3273427-3273020 [Escherichia coli Nissle 1917]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T47044 NZ_CP007799:c3273427-3273020 [Escherichia coli Nissle 1917]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT47044 WP_000354046.1 NZ_CP007799:c3273674-3273408 [Escherichia coli Nissle 1917]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT47044 NZ_CP007799:c3273674-3273408 [Escherichia coli Nissle 1917]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References