Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 559956..560574 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECOLIN_RS02740 Protein ID WP_001291435.1
Coordinates 559956..560174 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECOLIN_RS02745 Protein ID WP_000344800.1
Coordinates 560200..560574 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS02710 (ECOLIN_02635) 555244..555816 + 573 WP_000779831.1 YbaY family lipoprotein -
ECOLIN_RS02715 (ECOLIN_02640) 555847..556158 - 312 WP_000409908.1 MGMT family protein -
ECOLIN_RS02720 (ECOLIN_02645) 556537..556890 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECOLIN_RS02725 (ECOLIN_02650) 556932..558482 - 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECOLIN_RS02730 (ECOLIN_02655) 558646..559116 - 471 WP_001304825.1 YlaC family protein -
ECOLIN_RS02735 (ECOLIN_02660) 559232..559783 - 552 WP_000102539.1 maltose O-acetyltransferase -
ECOLIN_RS02740 (ECOLIN_02665) 559956..560174 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECOLIN_RS02745 (ECOLIN_02670) 560200..560574 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECOLIN_RS02750 (ECOLIN_02675) 561119..564268 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
ECOLIN_RS02755 (ECOLIN_02680) 564291..565484 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T47029 WP_001291435.1 NZ_CP007799:c560174-559956 [Escherichia coli Nissle 1917]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T47029 NZ_CP007799:c560174-559956 [Escherichia coli Nissle 1917]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT47029 WP_000344800.1 NZ_CP007799:c560574-560200 [Escherichia coli Nissle 1917]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT47029 NZ_CP007799:c560574-560200 [Escherichia coli Nissle 1917]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References