Detailed information of TA system TA047017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 862917..863537
• Replicon: chromosome
• Accession: NZ_CP007786
• Organism: Burkholderia cepacia strain DDS 7H-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DM42_RS08805
• Protein ID: WP_144446004.1
• Coordinates: 862917..863234 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DM42_RS08810
• Protein ID: WP_034202654.1
• Coordinates: 863238..863537 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM42_RS08795	859426..860859	-	1434	WP_006490987.1	aldehyde dehydrogenase family protein	-
DM42_RS08800	860888..862528	-	1641	WP_006490980.1	acetolactate synthase large subunit	-
DM42_RS08805	862917..863234	+	318	WP_144446004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DM42_RS08810	863238..863537	+	300	WP_034202654.1	putative addiction module antidote protein	Antitoxin
DM42_RS08815	863586..864872	-	1287	WP_006490986.1	DUF445 domain-containing protein	-
DM42_RS08820	864982..866202	-	1221	WP_034202655.1	FAD-dependent oxidoreductase	-
DM42_RS08825	866192..866518	-	327	WP_006496019.1	non-heme iron oxygenase ferredoxin subunit	-
DM42_RS08830	866554..867030	-	477	WP_006490977.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
DM42_RS08835	867046..868317	-	1272	WP_006496020.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (9-102)

Antitoxin

• Uncharacterized protein HI1420 (4-97)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11841.52 Da
• Isoelectric Point: 7.2769

>T47017 WP_144446004.1 NZ_CP007786:862917-863234 [Burkholderia cepacia]
MPYSPPAFSIRTTDVFDAWFAGLPDRLAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYDVRRETIWVILL
CGGDQSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T47017 NZ_CP007786:862917-863234 [Burkholderia cepacia]
ATGCCCTACAGTCCTCCTGCATTCAGCATCCGGACCACCGACGTCTTCGACGCCTGGTTTGCCGGCCTGCCCGATCGCCT
CGCCAAACGCCGCATTCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACTGGAAATCCGCCGGCTCCCCCG
TCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATGACGTGAGGCGCGAAACGATATGGGTCATCCTGCTG
TGCGGCGGCGATCAATCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGCGCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10644.12 Da
• Isoelectric Point: 6.9774

>AT47017 WP_034202654.1 NZ_CP007786:863238-863537 [Burkholderia cepacia]
MDKIKTRPWDSAEHLKTEDDMADYFDACLQEAGDDPAFIAHALGVIARARGMSQVARHAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHAAKAAA

Nucleotide

• Length: 300 bp

>AT47017 NZ_CP007786:863238-863537 [Burkholderia cepacia]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
ATGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCACGCGGCATGTCGC
AGGTCGCGCGCCATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTCATCAAGGCGCTCGGCCTGCAATTGCACGCCGCCAAGGCTGCCGCGTGA