Detailed information of TA system TA047016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 457517..458163
• Replicon: chromosome
• Accession: NZ_CP007786
• Organism: Burkholderia cepacia strain DDS 7H-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DM42_RS06960
• Protein ID: WP_034202503.1
• Coordinates: 457517..457924 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4EGU8
• Locus tag: DM42_RS06965
• Protein ID: WP_006491146.1
• Coordinates: 457921..458163 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM42_RS06945	453412..453993	-	582	WP_034202501.1	hypothetical protein	-
DM42_RS06950	453990..454718	-	729	WP_034203357.1	chemotaxis protein	-
DM42_RS06955	454741..457116	-	2376	WP_034202502.1	MCP four helix bundle domain-containing protein	-
DM42_RS06960	457517..457924	-	408	WP_034202503.1	type II toxin-antitoxin system VapC family toxin	Toxin
DM42_RS06965	457921..458163	-	243	WP_006491146.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DM42_RS06970	458422..459105	+	684	WP_034202504.1	phospholipase	-
DM42_RS06975	459179..460684	-	1506	WP_006495108.1	amino acid permease	-
DM42_RS06980	460762..462168	-	1407	WP_034202505.1	aspartate ammonia-lyase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14848.03 Da
• Isoelectric Point: 7.0738

>T47016 WP_034202503.1 NZ_CP007786:c457924-457517 [Burkholderia cepacia]
MKFLLDTNAVIAILKGEPAILARLHAWHPADFGIPAIVAHALYYGAYKSQRAAANVARVEALQFEVVSFDTGDARHAGEI
RAHLIAAGTPIGPYDALIAGQARARQLVLVTHNVREFERVPRLQFEDWLAEPRAD

Nucleotide

• Length: 408 bp

>T47016 NZ_CP007786:c457924-457517 [Burkholderia cepacia]
ATGAAGTTCCTGCTGGATACCAACGCGGTGATCGCCATTCTCAAAGGCGAGCCGGCGATACTGGCCCGTTTGCATGCCTG
GCACCCGGCCGATTTCGGGATCCCCGCGATCGTCGCGCACGCGCTTTACTACGGCGCGTACAAGAGCCAGCGAGCGGCGG
CGAACGTCGCGCGTGTCGAGGCGTTGCAGTTCGAGGTCGTATCATTCGACACCGGGGATGCACGGCATGCGGGTGAAATT
CGCGCGCACCTGATCGCGGCCGGCACGCCGATTGGTCCATACGACGCCTTGATCGCAGGGCAGGCGCGCGCGCGGCAACT
GGTGCTGGTCACGCACAACGTCCGCGAATTCGAGCGCGTTCCGCGGCTGCAATTCGAAGACTGGCTAGCCGAACCGAGGG
CAGACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.02 Da
• Isoelectric Point: 5.0197

>AT47016 WP_006491146.1 NZ_CP007786:c458163-457921 [Burkholderia cepacia]
MDTAKLFKHGGSQAVRLPKDFRFDTAEVRIRRHGAAVILEPMPQDWAWLAPLIGPVDADFEAAATTQPSAQERSGLDVFE

Nucleotide

• Length: 243 bp

>AT47016 NZ_CP007786:c458163-457921 [Burkholderia cepacia]
ATGGATACTGCAAAGCTCTTCAAGCATGGCGGCTCGCAGGCTGTCCGGCTGCCGAAGGATTTCCGCTTCGATACGGCCGA
GGTTCGCATCCGCCGCCACGGGGCGGCCGTCATTCTCGAGCCGATGCCGCAAGACTGGGCTTGGCTGGCACCGCTGATCG
GTCCGGTGGACGCCGATTTCGAGGCTGCCGCGACCACACAGCCGTCCGCTCAGGAGCGCTCGGGCCTGGACGTGTTCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: B4EGU8