Detailed information of TA system TA047005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1634791..1635393
• Replicon: chromosome
• Accession: NZ_CP007783
• Organism: Burkholderia cenocepacia strain DDS 22E-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A088U8B8
• Locus tag: DM39_RS11975
• Protein ID: WP_040127323.1
• Coordinates: 1635211..1635393 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A088UY23
• Locus tag: DM39_RS11970
• Protein ID: WP_040127322.1
• Coordinates: 1634791..1635186 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DM39_RS11925	1630544..1630849	+	306	WP_144397756.1	hypothetical protein	-
DM39_RS11930	1630846..1631409	+	564	WP_040127316.1	hypothetical protein	-
DM39_RS11935	1631409..1631741	+	333	WP_040127317.1	hypothetical protein	-
DM39_RS11940	1631738..1632181	+	444	WP_040127318.1	DUF1364 family protein	-
DM39_RS11945	1632235..1632516	+	282	WP_052102278.1	hypothetical protein	-
DM39_RS11950	1632513..1632761	+	249	WP_040127320.1	hypothetical protein	-
DM39_RS11955	1632803..1633222	+	420	WP_144397829.1	hypothetical protein	-
DM39_RS36175	1633226..1634242	+	1017	WP_144397757.1	hypothetical protein	-
DM39_RS11965	1634460..1634723	-	264	WP_040127321.1	hypothetical protein	-
DM39_RS11970	1634791..1635186	-	396	WP_040127322.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DM39_RS11975	1635211..1635393	-	183	WP_040127323.1	type II toxin-antitoxin system HicA family toxin	Toxin
DM39_RS36180	1635435..1635791	+	357	WP_040127324.1	HNH endonuclease	-
DM39_RS11985	1635956..1636432	+	477	WP_040127325.1	phage terminase small subunit P27 family	-
DM39_RS11990	1636436..1638151	+	1716	WP_040127326.1	terminase large subunit	-
DM39_RS11995	1638148..1638333	+	186	WP_040127327.1	hypothetical protein	-
DM39_RS12000	1638338..1639597	+	1260	WP_040127328.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1612798..1711843	99045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6803.00 Da
• Isoelectric Point: 10.9132

>T47005 WP_040127323.1 NZ_CP007783:c1635393-1635211 [Burkholderia cenocepacia]
MNSAELIKRIKADGWYHVHTVGSHHQFKHPTKPGKVTVPHPKKDLLIATVRSILKQADLK

Nucleotide

• Length: 183 bp

>T47005 NZ_CP007783:c1635393-1635211 [Burkholderia cenocepacia]
ATGAACAGCGCCGAACTCATCAAGCGAATTAAGGCCGATGGCTGGTACCACGTACATACCGTCGGGTCGCACCACCAGTT
CAAACACCCGACAAAGCCGGGCAAGGTAACGGTGCCTCACCCGAAGAAAGACCTGCTGATCGCTACGGTGCGCAGCATCC
TGAAACAAGCCGATCTGAAATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14331.12 Da
• Isoelectric Point: 4.7514

>AT47005 WP_040127322.1 NZ_CP007783:c1635186-1634791 [Burkholderia cenocepacia]
MLYPLYVHVGDAKHAHGVTFPDFPGCHAAADTWEDLPAAVQEAAEAHFYDDDGPVPAPSPLESLARNPEYEGGVWMIFDI
DLSKINSKAVRLNISLPERLVQQIDAAARARKLSRSAFLALAAEHEMAEHA

Nucleotide

• Length: 396 bp

>AT47005 NZ_CP007783:c1635186-1634791 [Burkholderia cenocepacia]
ATGCTCTATCCACTCTATGTCCACGTTGGCGACGCCAAGCATGCTCACGGCGTCACGTTTCCCGACTTCCCGGGCTGTCA
CGCAGCAGCCGATACCTGGGAAGATCTTCCCGCAGCCGTACAGGAAGCGGCCGAGGCGCATTTCTATGATGACGATGGCC
CGGTGCCAGCTCCGTCGCCGCTGGAATCACTTGCACGCAATCCCGAATACGAAGGCGGCGTCTGGATGATCTTCGATATT
GATCTGTCGAAGATCAACTCCAAGGCAGTTCGCCTCAATATCAGCCTGCCCGAACGGCTCGTGCAGCAGATCGATGCGGC
CGCTCGCGCACGCAAGCTGTCACGCTCCGCATTCCTCGCGCTTGCAGCAGAGCACGAAATGGCCGAACACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A088U8B8

Antitoxin

• AlphaFold DB: A0A088UY23